Two-Photon Microscopy Imaging Core

双光子显微成像核心

• 批准号: 7133534
• 负责人: OLGA KOVBASNJUK
• 金额: $ 9.16万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7133534
• 关键词: Internet; bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; experimental designs; fluorescence microscopy; fluorescence recovery after photobleaching; fluorescence resonance energy transfer; gastrointestinal imaging /visualization; information dissemination; molecular /cellular imaging; technology /technique development; training

Core C: The Two-Photon Microscopy and Confocal FRET/FRAP Imaging Core will: 1) Provide equipment and expertise to facilitate the use of the multiphoton microscopy for physiologic studies by PPG investigators. 2. Develop and apply advanced imaging techniques, especially FRET and FRAP on the MRC-1024MP, twophoton microscope and Gl Division confocal microscope (Zeiss 410). 3. Provide user training for imaging of cellular and tissue modes by multiphoton confocal microscopy. 4. Provide on-going support for experimental design, developing new techniques (now including FRET and FRAP), methods of analysis, and reviewing experimental results related to Core C equipment. 5. Maintain multiphoton microscope Web page/sign-up program. 6. Organize and maintain all equipment and usage records. 7. Provide data to Core A for charge-back of Core C use by non-PPG members of Hopkins community.

核心C：双光子显微镜和共焦FRET/FRAP成像核心将： 1)提供设备和专门知识，促进使用多光子显微镜进行生理学研究 由PPG调查人员。 2.开发和应用先进的成像技术，特别是在MRC-1024MP上的FRET和FRAP，双光子 显微镜和Gl分部共焦显微镜(蔡司410)。 3.提供多光子共聚焦显微镜对细胞和组织模式成像的用户培训。 4.为实验设计提供持续支持，开发新技术(现在包括FRET和 FRAP)，分析方法，以及回顾与核心C设备相关的实验结果。 维护多光子显微镜网页/注册程序。 6.整理和维护所有设备和使用记录。 7.向核心A提供数据，以供霍普金斯社区的非PPG成员按存储容量使用核心C。