Core B-Imaging
核心B超成像
基本信息
- 批准号:9279104
- 负责人:
- 金额:$ 21.3万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:AwardBasic ScienceCell modelCellsCellular biologyCollaborationsConfocal MicroscopyContract ServicesCountryDataData AnalysesDetectionDigestive System DisordersDiseaseDoctor of PhilosophyEducational process of instructingElectron MicroscopyEnsureEpithelial CellsEpithelial PhysiologyEquipmentEquipment FailureFee-for-Service PlansFloorFluorescenceFluorescence MicroscopyFluorescence Recovery After PhotobleachingFluorescence Resonance Energy TransferFundingFutureGoalsImageImaging TechniquesImaging technologyIn VitroIndustryInstructionIntestinesIonsKidneyLiverMeasurementMeasuresMethodologyMicroscopeMicroscopyMonitorMorphologyNational Institute of Diabetes and Digestive and Kidney DiseasesOrganOrganellesPancreasPhysiologyPreparationProductivityProgram Research Project GrantsProteinsPublicationsReaderResearchResearch PersonnelResolutionResourcesScanningServicesSpecialistSystemTechniquesTechnologyTimeTissue imagingTissuesTotal Internal Reflection FluorescentTrainingTranslational ResearchTransmission Electron MicroscopyUnited States National Institutes of HealthWagesWorkbasecharge coupled device cameracostdesignexperiencefluorophorehistological slidesimaging modalityimaging systemin vivo Modelmembermicroscopic imagingnew technologynovelprotein complexprotein protein interactionprotein transportratiometricspectrographtwo-photon
项目摘要
Imaging Core B: Project Summary
The DDBTRCC Imaging Core B is designed to provide our Research Base investigators. equipment for and
instruction and assistance in use of multiple levels of classical and cutting edge fluorescence microscopy and
electron microscopy (EM) techniques for study of the physiology and cell biology of GI track epithelial cells at
the level of organs, tissues, cell models, and sub-cellular organelles. These include confocal and multiphoton
confocal microscopy with applications for intracellular pH and Ca2+ measurements and protein trafficking at a
single cell level, FRET microscopy for detection of protein-protein interactions in in vitro and in vivo models,
FRAP microscopy for monitoring the changes in protein mobility due to the changes in protein complexes, and
META spectral imaging for more than 4 fluorophores. The Core also offers ratio-excitation imaging systems of
single cells (microscope/camera based) or groups of cells (fluorimeter) for quantitative measurements of ion
concentrations in polarized epithelial cells or in non-polarized cells and a cooled CCD camera for documenting
histologic slides. Core B has expanded the repertoire of microscopy imaging techniques by being awarded a
SIG (collaboration with JHU Imaging Core) to bring super resolution technology to JHU. Additionally, we
developed a partnership with industry and opened an Olympus Equipment Demonstration Center, to provide
access to Center members of advanced Olympus microscopy equipment which already includes confocal-in-a-
box technology for long time (days) live tissue imaging and advanced TIRF microscopy. Core B also assists
DDBTRCC investigators in preparation of intestine, liver, pancreas, and kidney for EM imaging and data
interpretation. Since the Center was funded in 2011, the Imaging Core has provided services to 44 current and
previous Center investigators, 37 Members and 7 Associate Members and contributed to 76 publications.
成像核心B:项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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OLGA KOVBASNJUK其他文献
OLGA KOVBASNJUK的其他文献
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{{ truncateString('OLGA KOVBASNJUK', 18)}}的其他基金
Role of Mucus in EHEC colonization of human colonoids and intestinal disease devleopment
粘液在肠出血性大肠杆菌定植于人类结肠和肠道疾病发展中的作用
- 批准号:
9351785 - 财政年份:2016
- 资助金额:
$ 21.3万 - 项目类别:
Multiphoton Laser Scanning Microscope: Zeiss LSM710 NLO
多光子激光扫描显微镜:Zeiss LSM710 NLO
- 批准号:
7793075 - 财政年份:2010
- 资助金额:
$ 21.3万 - 项目类别:
Shiga toxin uptake mechanisms and intracellular action
志贺毒素摄取机制和细胞内作用
- 批准号:
7934544 - 财政年份:2009
- 资助金额:
$ 21.3万 - 项目类别:
Shiga toxin uptake mechanisms and intracellular action
志贺毒素摄取机制和细胞内作用
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7654068 - 财政年份:2009
- 资助金额:
$ 21.3万 - 项目类别:
Zeiss 510/META Confocal Microscope Plus Live Cell Chamber
Zeiss 510/META 共焦显微镜 Plus 活细胞室
- 批准号:
7216564 - 财政年份:2007
- 资助金额:
$ 21.3万 - 项目类别:
Shiga toxin 1:uptake mechanisms and intracellular action
志贺毒素 1:摄取机制和细胞内作用
- 批准号:
6799566 - 财政年份:2003
- 资助金额:
$ 21.3万 - 项目类别:
Shiga toxin 1:uptake mechanisms and intracellular action
志贺毒素 1:摄取机制和细胞内作用
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6680759 - 财政年份:2003
- 资助金额:
$ 21.3万 - 项目类别:
Shiga toxin 1:uptake mechanisms and intracellular action
志贺毒素 1:摄取机制和细胞内作用
- 批准号:
6935381 - 财政年份:2003
- 资助金额:
$ 21.3万 - 项目类别:
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