Core B-Imaging

成像核心B：项目总结 DDBTRCC成像核心B旨在为我们的研究基地研究人员提供。设备和 指导和协助使用多层次的经典和尖端荧光显微镜， 电子显微镜（EM）技术用于研究胃肠道上皮细胞的生理学和细胞生物学， 器官、组织、细胞模型和亚细胞器水平。其中包括共焦和多光子 共聚焦显微镜用于细胞内pH和Ca 2+测量以及蛋白质运输 单细胞水平，FRET显微镜用于检测体外和体内模型中的蛋白质-蛋白质相互作用， FRAP显微镜用于监测由于蛋白质复合物的变化而引起的蛋白质迁移率的变化，以及 Meta光谱成像超过4个荧光团。Core还提供比率激励成像系统， 单细胞（基于显微镜/照相机）或细胞组（荧光计），用于离子的定量测量 极化上皮细胞或非极化细胞中的浓度，以及用于记录 组织切片。核心B扩大了显微成像技术的剧目，被授予 SIG（与JHU Imaging Core合作）为JHU带来超分辨率技术。另外我们 与工业界建立了合作伙伴关系，并开设了奥林巴斯设备演示中心， 访问先进的奥林巴斯显微镜设备的中心成员，其中已经包括共焦在一个， 盒技术用于长时间（数天）活组织成像和先进的TIRF显微镜。核心B也协助 DDBTRCC研究人员为EM成像和数据准备肠道、肝脏、胰腺和肾脏 解释。自2011年中心获得资助以来，成像核心已为44名现任和 前中心研究人员，37名成员和7名准成员，并为76篇出版物做出了贡献。

Imaging Core B: Project Summary The DDBTRCC Imaging Core B is designed to provide our Research Base investigators. equipment for and instruction and assistance in use of multiple levels of classical and cutting edge fluorescence microscopy and electron microscopy (EM) techniques for study of the physiology and cell biology of GI track epithelial cells at the level of organs, tissues, cell models, and sub-cellular organelles. These include confocal and multiphoton confocal microscopy with applications for intracellular pH and Ca2+ measurements and protein trafficking at a single cell level, FRET microscopy for detection of protein-protein interactions in in vitro and in vivo models, FRAP microscopy for monitoring the changes in protein mobility due to the changes in protein complexes, and META spectral imaging for more than 4 fluorophores. The Core also offers ratio-excitation imaging systems of single cells (microscope/camera based) or groups of cells (fluorimeter) for quantitative measurements of ion concentrations in polarized epithelial cells or in non-polarized cells and a cooled CCD camera for documenting histologic slides. Core B has expanded the repertoire of microscopy imaging techniques by being awarded a SIG (collaboration with JHU Imaging Core) to bring super resolution technology to JHU. Additionally, we developed a partnership with industry and opened an Olympus Equipment Demonstration Center, to provide access to Center members of advanced Olympus microscopy equipment which already includes confocal-in-a- box technology for long time (days) live tissue imaging and advanced TIRF microscopy. Core B also assists DDBTRCC investigators in preparation of intestine, liver, pancreas, and kidney for EM imaging and data interpretation. Since the Center was funded in 2011, the Imaging Core has provided services to 44 current and previous Center investigators, 37 Members and 7 Associate Members and contributed to 76 publications.