Core B-Imaging

核心B超成像

• 批准号: 9279104
• 负责人: OLGA KOVBASNJUK
• 金额: $ 21.3万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9279104
• 关键词: Award; Basic Science; Cell model; Cells; Cellular biology; Collaborations; Confocal Microscopy; Contract Services; Country; Data; Data Analyses; Detection; Digestive System Disorders; Disease; Doctor of Philosophy; Educational process of instructing; Electron Microscopy; Ensure; Epithelial Cells; Epithelial Physiology; Equipment; Equipment Failure; Fee-for-Service Plans; Floor; Fluorescence; Fluorescence Microscopy; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Funding; Future; Goals; Image; Imaging Techniques; Imaging technology; In Vitro; Industry; Instruction; Intestines; Ions; Kidney; Liver; Measurement; Measures; Methodology; Microscope; Microscopy; Monitor; Morphology; National Institute of Diabetes and Digestive and Kidney Diseases; Organ; Organelles; Pancreas; Physiology; Preparation; Productivity; Program Research Project Grants; Proteins; Publications; Reader; Research; Research Personnel; Resolution; Resources; Scanning; Services; Specialist; System; Techniques; Technology; Time; Tissue imaging; Tissues; Total Internal Reflection Fluorescent; Training; Translational Research; Transmission Electron Microscopy; United States National Institutes of Health; Wages; Work; base; charge coupled device camera; cost; design; experience; fluorophore; histological slides; imaging modality; imaging system; in vivo Model; member; microscopic imaging; new technology; novel; protein complex; protein protein interaction; protein transport; ratiometric; spectrograph; two-photon

Imaging Core B: Project Summary The DDBTRCC Imaging Core B is designed to provide our Research Base investigators. equipment for and instruction and assistance in use of multiple levels of classical and cutting edge fluorescence microscopy and electron microscopy (EM) techniques for study of the physiology and cell biology of GI track epithelial cells at the level of organs, tissues, cell models, and sub-cellular organelles. These include confocal and multiphoton confocal microscopy with applications for intracellular pH and Ca2+ measurements and protein trafficking at a single cell level, FRET microscopy for detection of protein-protein interactions in in vitro and in vivo models, FRAP microscopy for monitoring the changes in protein mobility due to the changes in protein complexes, and META spectral imaging for more than 4 fluorophores. The Core also offers ratio-excitation imaging systems of single cells (microscope/camera based) or groups of cells (fluorimeter) for quantitative measurements of ion concentrations in polarized epithelial cells or in non-polarized cells and a cooled CCD camera for documenting histologic slides. Core B has expanded the repertoire of microscopy imaging techniques by being awarded a SIG (collaboration with JHU Imaging Core) to bring super resolution technology to JHU. Additionally, we developed a partnership with industry and opened an Olympus Equipment Demonstration Center, to provide access to Center members of advanced Olympus microscopy equipment which already includes confocal-in-a- box technology for long time (days) live tissue imaging and advanced TIRF microscopy. Core B also assists DDBTRCC investigators in preparation of intestine, liver, pancreas, and kidney for EM imaging and data interpretation. Since the Center was funded in 2011, the Imaging Core has provided services to 44 current and previous Center investigators, 37 Members and 7 Associate Members and contributed to 76 publications.

成像核心 B：项目摘要 DDBTRCC 成像核心 B 旨在为我们的研究基地研究人员提供服务。设备和 使用多级经典和尖端荧光显微镜的指导和帮助 电子显微镜（EM）技术用于研究胃肠道上皮细胞的生理学和细胞生物学 器官、组织、细胞模型和亚细胞器的水平。这些包括共焦和多光子 共聚焦显微镜可用于细胞内 pH 和 Ca2+ 测量以及蛋白质运输 单细胞水平，FRET 显微镜用于检测体外和体内模型中蛋白质-蛋白质相互作用， FRAP 显微镜用于监测由于蛋白质复合物的变化而导致的蛋白质迁移率的变化，以及 适用于 4 个以上荧光团的 META 光谱成像。 Core 还提供比率激发成像系统 单细胞（基于显微镜/相机）或细胞组（荧光计）用于离子的定量测量 偏振上皮细胞或非偏振细胞中的浓度以及用于记录的冷却 CCD 相机 组织学幻灯片。 Core B 获得了 SIG（与 JHU Imaging Core 合作）将超分辨率技术引入 JHU。此外，我们 与业界建立了合作伙伴关系并开设了奥林巴斯设备演示中心，以提供 访问先进的奥林巴斯显微镜设备的中心成员，其中已经包括共焦- 用于长时间（数天）活体组织成像的盒子技术和先进的 TIRF 显微镜。核心B也有协助 DDBTRCC 研究人员正在准备肠道、肝脏、胰腺和肾脏以进行 EM 成像和数据 解释。自 2011 年资助该中心以来，成像核心已为 44 家现有和 前中心调查员、37 名会员和 7 名准会员，并发表了 76 篇出版物。