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Core B-Imaging

核心B超成像

基本信息

批准号：
9279104
负责人：
OLGA KOVBASNJUK
金额：
$ 21.3万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9279104
关键词：
Award Basic Science Cell model Cells Cellular biology Collaborations Confocal Microscopy Contract Services Country Data Data Analyses Detection Digestive System Disorders Disease Doctor of Philosophy Educational process of instructing Electron Microscopy Ensure Epithelial Cells Epithelial Physiology Equipment Equipment Failure Fee-for-Service Plans Floor Fluorescence Fluorescence Microscopy Fluorescence Recovery After Photobleaching Fluorescence Resonance Energy Transfer Funding Future Goals Image Imaging Techniques Imaging technology In Vitro Industry Instruction Intestines Ions Kidney Liver Measurement Measures Methodology Microscope Microscopy Monitor Morphology National Institute of Diabetes and Digestive and Kidney Diseases Organ Organelles Pancreas Physiology Preparation Productivity Program Research Project Grants Proteins Publications Reader Research Research Personnel Resolution Resources Scanning Services Specialist System Techniques Technology Time Tissue imaging Tissues Total Internal Reflection Fluorescent Training Translational Research Transmission Electron Microscopy United States National Institutes of Health Wages Work base charge coupled device camera cost design experience fluorophore histological slides imaging modality imaging system in vivo Model member microscopic imaging new technology novel protein complex protein protein interaction protein transport ratiometric spectrograph two-photon

项目摘要

Imaging Core B: Project Summary The DDBTRCC Imaging Core B is designed to provide our Research Base investigators. equipment for and instruction and assistance in use of multiple levels of classical and cutting edge fluorescence microscopy and electron microscopy (EM) techniques for study of the physiology and cell biology of GI track epithelial cells at the level of organs, tissues, cell models, and sub-cellular organelles. These include confocal and multiphoton confocal microscopy with applications for intracellular pH and Ca2+ measurements and protein trafficking at a single cell level, FRET microscopy for detection of protein-protein interactions in in vitro and in vivo models, FRAP microscopy for monitoring the changes in protein mobility due to the changes in protein complexes, and META spectral imaging for more than 4 fluorophores. The Core also offers ratio-excitation imaging systems of single cells (microscope/camera based) or groups of cells (fluorimeter) for quantitative measurements of ion concentrations in polarized epithelial cells or in non-polarized cells and a cooled CCD camera for documenting histologic slides. Core B has expanded the repertoire of microscopy imaging techniques by being awarded a SIG (collaboration with JHU Imaging Core) to bring super resolution technology to JHU. Additionally, we developed a partnership with industry and opened an Olympus Equipment Demonstration Center, to provide access to Center members of advanced Olympus microscopy equipment which already includes confocal-in-a- box technology for long time (days) live tissue imaging and advanced TIRF microscopy. Core B also assists DDBTRCC investigators in preparation of intestine, liver, pancreas, and kidney for EM imaging and data interpretation. Since the Center was funded in 2011, the Imaging Core has provided services to 44 current and previous Center investigators, 37 Members and 7 Associate Members and contributed to 76 publications.

成像核心B：项目总结