Core B-Imaging

成像核心B：项目摘要 DDBTRCC成像核心B旨在为我们的研究基地调查人员提供。和的设备 指导和协助使用多层次的经典和尖端荧光显微镜和 电子显微镜(EM)技术研究胃肠道径路上皮细胞的生理和细胞生物学 器官、组织、细胞模型和亚细胞细胞器的水平。其中包括共焦和多光子 共聚焦显微镜及其在细胞内pH和钙离子测量和蛋白质转运中的应用 单细胞水平，用于检测体外和体内模型中蛋白质-蛋白质相互作用的FRET显微镜， FRAP显微镜用于监测由于蛋白质复合体的变化而引起的蛋白质迁移率的变化，以及 4个以上荧光团的偏光谱成像。酷睿还提供比率激励成像系统 用于离子定量测量的单个细胞(基于显微镜/照相机)或细胞组(荧光计) 极化上皮细胞或非极化细胞中的浓度和用于记录的冷却CCD相机 组织学切片。核心B扩展了显微成像技术的曲目，获得了 SIG(与JHU成像核心合作)为JHU带来超分辨率技术。此外，我们 与业界建立了合作伙伴关系，并开设了奥林巴斯设备演示中心，以提供 接触到先进的奥林巴斯显微镜设备的中心成员，这些设备已经包括共焦在a-a- BOX技术可用于长时间(数天)的活体组织成像和先进的TIRF显微镜。核心B也协助 DDBTRCC研究人员正在为EM成像和数据准备肠、肝、胰腺和肾脏 释义。自2011年为该中心提供资金以来，成像核心已向44个现有和 以前的中心调查人员，37名成员和7名准成员，并为76份出版物作出贡献。

Imaging Core B: Project Summary The DDBTRCC Imaging Core B is designed to provide our Research Base investigators. equipment for and instruction and assistance in use of multiple levels of classical and cutting edge fluorescence microscopy and electron microscopy (EM) techniques for study of the physiology and cell biology of GI track epithelial cells at the level of organs, tissues, cell models, and sub-cellular organelles. These include confocal and multiphoton confocal microscopy with applications for intracellular pH and Ca2+ measurements and protein trafficking at a single cell level, FRET microscopy for detection of protein-protein interactions in in vitro and in vivo models, FRAP microscopy for monitoring the changes in protein mobility due to the changes in protein complexes, and META spectral imaging for more than 4 fluorophores. The Core also offers ratio-excitation imaging systems of single cells (microscope/camera based) or groups of cells (fluorimeter) for quantitative measurements of ion concentrations in polarized epithelial cells or in non-polarized cells and a cooled CCD camera for documenting histologic slides. Core B has expanded the repertoire of microscopy imaging techniques by being awarded a SIG (collaboration with JHU Imaging Core) to bring super resolution technology to JHU. Additionally, we developed a partnership with industry and opened an Olympus Equipment Demonstration Center, to provide access to Center members of advanced Olympus microscopy equipment which already includes confocal-in-a- box technology for long time (days) live tissue imaging and advanced TIRF microscopy. Core B also assists DDBTRCC investigators in preparation of intestine, liver, pancreas, and kidney for EM imaging and data interpretation. Since the Center was funded in 2011, the Imaging Core has provided services to 44 current and previous Center investigators, 37 Members and 7 Associate Members and contributed to 76 publications.