Multiphoton Laser Scanning Microscope: Zeiss LSM710 NLO

多光子激光扫描显微镜：Zeiss LSM710 NLO

• 批准号: 7793075
• 负责人: OLGA KOVBASNJUK
• 金额: $ 49.85万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2011-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793075
• 关键词: Area; Award; Back; Cardiology; Center Core Grants; Charge; Communities; Contract Services; Disease; Dyes; Energy Transfer; Equipment Malfunction; Fluorescence Recovery After Photobleaching; Funding; Generations; Grant; Image; Immunofluorescence Immunologic; Laboratories; Lasers; Malignant Neoplasms; Microscope; Microscopy; Minor; National Heart, Lung, and Blood Institute; National Institute of Diabetes and Digestive and Kidney Diseases; Physics; Protocols documentation; Research; Scanning; Scientist; Services; System; Tissues; Training; Tsunami; United States National Institutes of Health; detector; instrument; second harmonic

DESCRIPTION (provided by applicant): We request funds to purchase a multiphoton laser scanning microscope (Zeiss LSM 710 NLO) as a replacement for our outdated BioRad MRC-1024 MP which we have had since 1999. Studies using this instrument were a key to us obtaining two PPG grants (NIDDK and NHLBI), a NIDDK GI Core center grant, and several RO1s, all of which are ongoing. Service of all BioRad multiphoton instruments have been discontinued for the last 3 years and parts for the microscope, scanner, detectors and our old generation of Tsunami laser for multiphoton excitation (Spectral Physics) have been discontinued as well. Replacement of this instrument is a necessary step to assure the successful experimental continuity of these NIH awards. This microscope has 4 major and 3 minor users in three broad research areas: diarrheal diseases, cancer and cardiology. All of the major and minor users have funded NIH projects, the aims of which require use of a multiphoton microscope to accomplish the specific aims. As in the case of the current instrument, the proposed multiphoton system will be a part of the Ross Research Building Fluorescent Microscopy Core which is part of an NIH/NIDDK GI Core Center, of which Dr. Donowitz is the PI and Dr. Kovbasnjuk is the Imaging Core Co-Director and both of whom are major users. This Ross Microscopy Core (://www.hopkinsmedicine.org/confocal/) has provided instruments and training for Hopkins scientists for 15 years and has been used by over 100 laboratories at JHUSOM, including advanced imaging applications which include Forster Resonance Energy Transfer (FRET), Fluorescence Recovery After Photobleaching (FRAP), deconvolution, Second Harmonic Generation (SHG), quantitative imaging protocols for pH-sensitive SNARF-4Fdye, Ca indicator dyes, NAD(P)H, GFP, as well as deep tissue immunofluorescence protocols, etc. User charges in part along with NIH funding support the service contracts for the multiphoton as well as our new Zeiss LSM 510/META confocal system and for a Core Manager who oversees both administrative and scientific Core activities. Institutional support provides back up in case of NIH funding lapses or major equipment malfunction. The proposed instrument will be only the second system at Hopkins available for the general JHUSOM research community.

描述（由申请人提供）：我们请求资金购买多光子激光扫描显微镜 (Zeiss LSM 710 NLO)，以替代我们自 1999 年以来一直使用的过时的 BioRad MRC-1024 MP。使用该仪器的研究是我们获得两项 PPG 资助（NIDDK 和 NHLBI）、一项 NIDDK GI 核心中心资助和几项 RO1 的关键，所有这些都是 正在进行中。所有 BioRad 多光子仪器的服务已在过去 3 年停止，显微镜、扫描仪、探测器和用于多光子激发（光谱物理）的老一代 Tsunami 激光器的零件也已停止。更换该仪器是确保这些 NIH 奖项实验成功连续性的必要步骤。该显微镜在三个广泛的研究领域有 4 个主要用户和 3 个次要用户：腹泻病、癌症和心脏病学。所有主要和次要用户都资助了 NIH 项目，其目标需要使用多光子显微镜来实现特定目标。与当前仪器的情况一样，拟议的多光子系统将成为罗斯研究大楼荧光显微镜核心的一部分，该核心是 NIH/NIDDK GI 核心中心的一部分，Donowitz 博士是该中心的 PI，Kovbasnjuk 博士是成像核心联合主任，两人都是主要用户。 Ross 显微镜核心 (://www.hopkinsmedicine.org/confocal/) 已为霍普金斯大学的科学家提供仪器和培训 15 年，并已被 JHUSOM 的 100 多个实验室使用，包括先进的成像应用，包括福斯特共振能量转移 (FRET)、光漂白后荧光恢复 (FRAP)、反卷积、二次谐波生成 (SHG)、定量成像协议 用于 pH 敏感的 SNARF-4Fdye、Ca 指示剂染料、NAD(P)H、GFP 以及深层组织免疫荧光协议等。用户费用与 NIH 资金一起支持多光子以及我们新的 Zeiss LSM 510/META 共焦系统以及监督行政和科学核心活动的核心经理的服务合同。机构支持可以在 NIH 资金失效或主要设备故障的情况下提供支持。拟议的仪器将是霍普金斯大学为一般 JHUSOM 研究界提供的第二个系统。