Multiphoton Laser Scanning Microscope: Zeiss LSM710 NLO

多光子激光扫描显微镜：Zeiss LSM710 NLO

• 批准号: 7793075
• 负责人: OLGA KOVBASNJUK
• 金额: $ 49.85万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2011-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793075
• 关键词: Area; Award; Back; Cardiology; Center Core Grants; Charge; Communities; Contract Services; Disease; Dyes; Energy Transfer; Equipment Malfunction; Fluorescence Recovery After Photobleaching; Funding; Generations; Grant; Image; Immunofluorescence Immunologic; Laboratories; Lasers; Malignant Neoplasms; Microscope; Microscopy; Minor; National Heart, Lung, and Blood Institute; National Institute of Diabetes and Digestive and Kidney Diseases; Physics; Protocols documentation; Research; Scanning; Scientist; Services; System; Tissues; Training; Tsunami; United States National Institutes of Health; detector; instrument; second harmonic

DESCRIPTION (provided by applicant): We request funds to purchase a multiphoton laser scanning microscope (Zeiss LSM 710 NLO) as a replacement for our outdated BioRad MRC-1024 MP which we have had since 1999. Studies using this instrument were a key to us obtaining two PPG grants (NIDDK and NHLBI), a NIDDK GI Core center grant, and several RO1s, all of which are ongoing. Service of all BioRad multiphoton instruments have been discontinued for the last 3 years and parts for the microscope, scanner, detectors and our old generation of Tsunami laser for multiphoton excitation (Spectral Physics) have been discontinued as well. Replacement of this instrument is a necessary step to assure the successful experimental continuity of these NIH awards. This microscope has 4 major and 3 minor users in three broad research areas: diarrheal diseases, cancer and cardiology. All of the major and minor users have funded NIH projects, the aims of which require use of a multiphoton microscope to accomplish the specific aims. As in the case of the current instrument, the proposed multiphoton system will be a part of the Ross Research Building Fluorescent Microscopy Core which is part of an NIH/NIDDK GI Core Center, of which Dr. Donowitz is the PI and Dr. Kovbasnjuk is the Imaging Core Co-Director and both of whom are major users. This Ross Microscopy Core (://www.hopkinsmedicine.org/confocal/) has provided instruments and training for Hopkins scientists for 15 years and has been used by over 100 laboratories at JHUSOM, including advanced imaging applications which include Forster Resonance Energy Transfer (FRET), Fluorescence Recovery After Photobleaching (FRAP), deconvolution, Second Harmonic Generation (SHG), quantitative imaging protocols for pH-sensitive SNARF-4Fdye, Ca indicator dyes, NAD(P)H, GFP, as well as deep tissue immunofluorescence protocols, etc. User charges in part along with NIH funding support the service contracts for the multiphoton as well as our new Zeiss LSM 510/META confocal system and for a Core Manager who oversees both administrative and scientific Core activities. Institutional support provides back up in case of NIH funding lapses or major equipment malfunction. The proposed instrument will be only the second system at Hopkins available for the general JHUSOM research community.

描述（由申请人提供）：我们要求资金购买多光子激光扫描显微镜（蔡司LSM 710 NLO），以取代我们自1999年以来已经过时的BioRad MRC-1024 MP。使用该仪器的研究是我们获得两项PPG拨款（NIDDK和NHLBI），一项NIDDK GI核心中心拨款和几个ro1的关键，所有这些都在进行中。所有BioRad多光子仪器的服务在过去3年已经停止，显微镜、扫描仪、探测器和我们的老一代多光子激发（光谱物理）海啸激光器的部件也已经停止。更换该仪器是确保这些NIH奖励成功的实验连续性的必要步骤。该显微镜在三个广泛的研究领域有4个主要用户和3个次要用户：腹泻病、癌症和心脏病学。所有的主要和次要用户都资助了NIH的项目，这些项目的目标需要使用多光子显微镜来完成特定的目标。就目前的仪器而言，拟议的多光子系统将成为罗斯研究大楼荧光显微镜核心的一部分，该核心是NIH/NIDDK GI核心中心的一部分，Donowitz博士是PI， Kovbasnjuk博士是成像核心的联合主任，他们都是主要用户。这个罗斯显微镜核心（://www.hopkinsmedicine.org/confocal/）为霍普金斯大学的科学家提供了15年的仪器和培训，并已被JHUSOM的100多个实验室使用，包括先进的成像应用，包括福斯特共振能量转移（FRET），光漂白后荧光恢复（FRAP），反卷积，二次谐波产生（SHG）， ph敏感的sngf - 4fdye， Ca指示染料，NAD(P)H， GFP，以及深层组织免疫荧光协议等。用户费用部分与NIH资助一起支持多光子服务合同以及我们新的蔡司LSM 510/META共聚焦系统，以及监督行政和科学核心活动的核心经理。机构支持在NIH资金失效或重大设备故障的情况下提供支持。拟议的仪器将是霍普金斯大学JHUSOM研究界可使用的第二个系统。