Zeiss 510/META Confocal Microscope Plus Live Cell Chamber

Zeiss 510/META 共焦显微镜 Plus 活细胞室

• 批准号: 7216564
• 负责人: OLGA KOVBASNJUK
• 金额: $ 44.42万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7216564
• 关键词: 11 year old; Arts; Basic Science; Carbon Dioxide; Cells; Computer software; Computers; Core Facility; Deterioration; Development; Digestive System Disorders; Doctor of Medicine; Doctor of Philosophy; Fee-for-Service Plans; Fluorescence Resonance Energy Transfer; Funding; Housing; Image; Laboratories; Lasers; Life; Location; Microscope; Minor; Modeling; Monoclonal Antibody R24; National Institute of Diabetes and Digestive and Kidney Diseases; Other Resources; Physiological; Request for Applications; Research; Research Personnel; Scanning; Scientist; Speed; System; Technology; Temperature; Time; United States National Institutes of Health; Week; base; cellular imaging; computerized data processing; fluorescence imaging; improved; instrument; prevent; research facility

DESCRIPTION (provided by applicant): This application requests a Zeiss 510/META laser scanning confocal microscope with environmental chamber to replace our 11 year old Zeiss 410 microscope, which is part of the Imaging Core (Core Director, O. Kovbasnjuk, Ph.D., who is PI of this application) of an NIH NIDDK Core Development Center (R24, DK064388, "Hopkins Basic Science Digestive Diseases Core Center", PI, M. Donowitz, M.D.). The Zeiss 510/META microscope will allow us to provide state-of-the-art fluorescence imaging capabilities for 5 major interacting investigators, who are supported by the NIH NIDDK Core Development Center and who are closely interacting. There are also 6 minor users supported by the same Core Center who have their laboratories in the Ross Research Building, which houses the GI Core Center and the Zeiss confocal. In addition to being part of an NIH funded Core Center, the 510/META will be a resource for other JHUSOM scientists needing an environmental chamber on a confocal and will be available on a fee-for-service basis to scientists in a major Hopkins research facility (Ross Building). The 410 no longer satisfies our users' needs which can be met by the 510/META. This is due to 1) Deterioration of the Zeiss 410 PMT's and pin holes plus its outmoded software and tenuous computer support so the entire system will not be functional once the current computer fails. 2) Lack of spectral scanning capability needed for FRET applications. 3) Need for extensive use of FRET and FRAP with improved precision on 510 due to more precise pixel alignment than 410. The features of the Zeiss 510/META which are necessary to accomplish the diverse biomedical projects and which do not exist on the Zeiss 410 model to be replaced include: multitracking technology for speed and precise pixel localization; Zeiss LSM510 and Axiovision software for rapid signal processing and analysis; and a live cell chamber with temperature and CO2 controls needed for prolonged live cell imaging. The JHUSOM Imaging Core is not able to provide adequate time for the physiologic studies proposed in the R24 Center. This is preventing the proposed FRET studies and overnight constant temperature chamber studies. Importantly, no instruments available on a fee-for-service basis at JHUSOM are equipped with a live cell chamber. The Zeiss 510/META will be installed in the NIH NIDDK Core Center Imaging Core Facility, a location that is central to most of the users. It is anticipated that 75% of the 40 hr week will be used by the 5 major plus 6 minor users, with the remaining 25%, representing ~10-15 additional hrs/week, will be taken up by fee-for-service users, mostly from the Ross Building plus other Hopkins users needing the live cell chamber.

描述（由申请人提供）：此申请要求Zeiss 510/Meta Laser扫描共凝结显微镜与环境室取代我们11岁的Zeiss 410显微镜，该显微镜是Imaging Core的一部分（核心导演，O。Kovbasnjuk，O。Kovbasnjuk，Ph.D。消化疾病核心中心”，PI，M。Donowitz，M.D。）。 Zeiss 510/Meta显微镜将使我们能够为5个主要相互作用的研究人员提供最先进的荧光成像能力，这些研究人员得到了NIH NIDDK核心发展中心的支持，并且紧密相互作用。同一核心中心也有6个未成年人支持，他们在Ross Research大楼中设有实验室，该大楼设有GI Core Center和Zeiss Congocal。除了成为NIH资助的核心中心的一部分外，510/META还将成为其他需要共享环境室的Jhusom科学家的资源，并将在霍普金斯大型研究设施（Ross Buildity）中的科学家提供收费的基础上提供。 410不再满足我们用户的需求，而510/meta可以满足我们的需求。这是由于1）Zeiss 410 PMT和引脚孔的恶化加上其过时的软件和差量的计算机支持，因此一旦当前计算机失败，整个系统将无法实现。 2）缺乏FRET应用所需的光谱扫描功能。 3）需要广泛使用FRET和FRAP，其精确度比410的精确像素对齐，并提高了精度。Zeiss 510/Meta的特征是完成多样化的生物医学项目所必需的，并且在Zeiss 410模型上不存在以替代的Zeiss 410不存在的功能，包括：多级技术用于速度和精确的Pixel Pixel定位； Zeiss LSM510和用于快速信号处理和分析的Axiovision软件；以及具有温度和二氧化碳对照的活细胞室，需要长时间的活细胞成像。 Jhusom成像核无法为R24中心提出的生理研究提供足够的时间。这是防止提出的fRET研究和隔夜恒温室研究。重要的是，JHUSOM的收费基础上没有可用的工具，配备了活电池室​​。 Zeiss 510/META将安装在NIH NIDDK核心中心成像核心设施中，这是大多数用户中心的位置。预计，在40个小时周中，有75％将由5个专业的6个未成年人使用，其余的25％（代表约10-15个小时/周）将由费用服务的用户占用，主要来自Ross Building，主要来自其他需要现场电池室的Hopkins用户。