Exploring otoacoustic emissions with intracochlear pressure measurements

通过耳蜗内压力测量探索耳声发射

• 批准号: 7320537
• 负责人: Wei Dong
• 金额: $ 8.05万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7320537
• 关键词: Auditory; Basilar Membrane; Categories; Cells; Characteristics; Clinic; Clinical Research; Cochlea; Diagnosis; Ear; Evaluation; External auditory canal; Frequencies; Generations; Gerbils; Hearing; Infant; Labyrinth; Lead; Light; Link; Liquid substance; Location; Measurement; Measures; Mechanics; Motion; Non-linear Models; Numbers; Patients; Personal Satisfaction; Phase; Positioning Attribute; Principal Investigator; Process; Protocols documentation; Range; Relative (related person); Research; Role; Scala Tympani; Series; Source; Stapes; Stimulus; Structure; System; Techniques; Testing; Title; Travel; Variant; Work; base; hearing impairment; human SLC25A5 protein; improved; in vivo; middle ear; otoacoustic emission; pressure; programs; research study; response; sensor; sound; theories; transmission process

DESCRIPTION (provided by applicant): This application aims to further investigate the important issue of how sound energy travels out of the cochlea and to provide a bridge between cochlear mechanics and otoacoustic emissions. In addition to perceiving sound, the ear also makes sound, as a result of the cell-based cochlear nonlinearity. Exactly how this energy radiates from the inner ear has been a major unresolved question since the discovery of these so-called "otoacoustic emissions" in 1976. This application will use the unique and powerful micro-pressure-sensor to probe the cochlear mechanics and a sensitive microphone system as the receiver to calibrate sound and measure the sound pressure in the ear canal. Direct and simultaneous measurements of the intracochlear pressure and ear canal pressures to sound stimuli will be performed in gerbil normal ears in vivo. Cochlear distortion products (DPs) will be used as the ideal intracochlear sound sources of the distortion product otoacoustic emissions (DPOAEs) being detected in the ear canal. The intracochlear approach will be at the basal turn of cochlea, where the cochlear mechanism has been well established in the field and also in the lab. By directly comparing the DPOAEs and DPs, the sound transmission in reverse direction will be further explored with the specific aims to category the role of the reverse traveling wave and the cochlear fluid, the cochlear nonlinearity and the contributions of DPs 'generator' & 'reflector' components to DPOAEs. The measurements will significantly improve our understanding of how the cochlea is excited by and produces sound and how the sound travels out of the cochlea. These sound emissions from the ear are widely used in clinic to detect and diagnose forms of hearing impairment, especially in infants and other patients whose hearing cannot be tested in other ways. Understanding the mechanisms of their generation and transmission is therefore crucial in many clinical and research applications. How exactly sound energy radiates from the inner ear has been a major unresolved question since the discovery of otoacoustic emissions (OAEs) in 1978 by Kemp. In addition to perceiving sound, the cochlea also generates sound, as a result of nonlinear cell-based forces. These 'cochlea-generated' sounds, known as OAEs being detected in the ear canal, are a noninvasive probe used in the clinic to detect and diagnose hearing impairment. Understanding the mechanisms of their generation and transmission is therefore crucial to many clinical and research applications. Recently a simultaneous recording technique of intracochlear pressure and EC pressure in gerbils has been well established in the lab, which has proven to be very illuminating for understanding cochlear mechanics. This proposal aims to use this combined studies of OAEs and intracochlear pressure to probe important aspects of the emission process in normal gerbil cochlea in vivo. The results of this project will lead to further understanding on the specific questions of (1) the intracochlear path OAEs take and (2) the possibility for amplification as these sounds travel out of the cochlea, and (3) the presence of different OAE components.

描述（由申请人提供）：本申请旨在进一步研究声能如何传播出耳蜗的重要问题，并在耳蜗力学和耳声发射之间提供桥梁。除了感知声音之外，由于基于细胞的耳蜗非线性，耳朵也发出声音。自从1976年发现所谓的“耳声发射”以来，这种能量究竟是如何从内耳辐射出来的一直是一个悬而未决的问题。该应用将使用独特而强大的微压力传感器来探测耳蜗力学，并使用灵敏的麦克风系统作为接收器来校准声音并测量耳道中的声压。本实验将在活体条件下对正常沙鼠耳进行声刺激的耳内压和耳道压的直接同步测量。相干失真产物（DP）将被用作耳道中检测的失真产物耳声发射（DPOAE）的理想的相干声源。耳蜗内入路将在耳蜗的基底回处，耳蜗机制已在现场和实验室中得到很好的建立。通过直接比较DPOAE和DP，将进一步探索反向的声音传输，具体目的是分类反向行波和耳蜗流体的作用，耳蜗非线性和DP的“发生器”和“反射器”组件对DPOAE的贡献。这些测量将大大提高我们对耳蜗如何被声音激发和产生声音以及声音如何从耳蜗传播出去的理解。这些来自耳朵的声音发射在临床上广泛用于检测和诊断各种形式的听力障碍，特别是对于婴儿和其他无法以其他方式测试听力的患者。因此，了解它们的产生和传播机制在许多临床和研究应用中至关重要。 自1978年肯普发现耳声发射（OAEs）以来，声能究竟如何从内耳辐射一直是一个悬而未决的问题。除了感知声音之外，由于非线性的细胞力，耳蜗还产生声音。这些“耳蜗产生”的声音，被称为耳道中检测到的OAE，是临床上用于检测和诊断听力障碍的非侵入性探头。因此，了解它们的产生和传播机制对许多临床和研究应用至关重要。近年来，在实验室中建立了一种同时记录沙土鼠耳蜗内压和EC压的技术，这对理解耳蜗力学具有重要意义。本研究旨在利用耳声发射和耳蜗内压的联合研究来探讨正常沙土鼠耳蜗在体发射过程的重要方面。 该项目的结果将导致对以下具体问题的进一步理解：（1）耳声发射的耳蜗内路径;（2）当这些声音从耳蜗传出时放大的可能性;以及（3）不同耳声发射成分的存在。