Antimicrobial Dental Devices and Waterlines

抗菌牙科设备和水管

• 批准号: 7326531
• 负责人: Ted W Reid
• 金额: $ 14.96万
• 依托单位: SELENIUM, LTD
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-15 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7326531
• 关键词: Acquired Immunodeficiency Syndrome; Adherence; Advocate; American Dental Association; Anti-Bacterial Agents; Antibiotics; Bacteria; Bacterial Adhesion; Bacteriocides; Binding; Biocompatible Coated Materials; Cardiac; Cell Death; Cell model; Cells; Centers for Disease Control and Prevention (U.S.); Chemicals; Child; Clinical; Collaborations; Daily; Dental; Dental Care; Dental General Practice; Dentistry; Development; Devices; Diabetes Mellitus; Disinfectants; Disinfection; Dose; Elderly; Enterococcus; Environment; Extracellular Matrix; Flushing; Genus Mycobacterium; Genus staphylococcus; Immune system; Immunocompromised Host; In Vitro; Infection; Infection Control; Klebsiella; Legionella; Maintenance; Malignant Neoplasms; Measures; Metabolic; Methods; Microbial Biofilms; Operative Surgical Procedures; Patients; Phase; Polyurethanes; Pregnant Women; Procedures; Process; Pseudomonas; Pseudomonas aeruginosa; Research; Safety; Sampling; Selenium; Small Business Technology Transfer Research; Smoker; Solutions; Staphylococcus aureus; Superoxides; Surface; Testing; Time; Water; Water Pollution; air filter; antimicrobial; base; burden of illness; chemical binding; day; density; killings; older patient; prevent; problem drinker; prophylactic; purge; research study; response; water flow

DESCRIPTION (provided by applicant): Bacterial contamination of Dental water lines is a major concern in modern Dentistry and may cause infection in Dental patients with compromised immune systems. This includes patients with cancer, diabetes, or AIDS; cardiac patients who often require prophylactic antibiotics prior to Dental treatment; the elderly, young children, and pregnant women. Bacterial levels in Dental water lines can reach 5000 times the American Dental Association's recommended upper limit for safety. Both the American Dental Association and the Centers for Disease Control advocate regular infection control procedures to disinfect the waterlines. Current disinfection methods (prolonged flushing, daily draining, filters, and chemical disinfectants) require regular maintenance and application, which may be impracticable in a busy Dental practice. Bacteria often reside in a biofilm, a dense slimy extracellular matrix in where the majority of bacteria are in a dormant metabolic state. These dormant bacteria are often unaffected by conventional antibiotics. Regular Dental line disinfectants rarely remove the biofilm. A more compelling solution would be to prevent biofilm formation in the first place. In this Phase I STTR project, we will examine the feasibility of a selenium-based antibacterial coating for the lumen of Dental waterlines. Selenium acts as a catalytic generator of superoxide radicals, which kill the bacteria. Selenium can be covalently bound to the interior of the water line tubing and can potentially permanently inhibit biofilm development. We will develop coating methods for polyurethane Dental tubing and test tubing samples for coating continuity and stability. We will also determine the relationship between coating density and antimicrobial efficacy. Finally, we will assess the efficacy of the coated tubing in flow cell experiments using Pseudomonas aeruginosa.

描述（由申请人提供）：牙科用水管线的细菌污染是现代牙科的一个主要问题，可能会导致免疫系统受损的牙科患者感染。这包括患有癌症、糖尿病或艾滋病的患者；在牙科治疗前经常需要预防性使用抗生素的心脏病患者；老人、幼儿和孕妇。牙科用水管线中的细菌含量可达到美国牙科协会建议的安全上限的 5000 倍。美国牙科协会和疾病控制中心都提倡定期进行感染控制程序，对水管进行消毒。目前的消毒方法（长时间冲洗、每日排水、过滤器和化学消毒剂）需要定期维护和使用，这在繁忙的牙科诊所中可能不切实际。细菌通常驻留在生物膜中，生物膜是一种致密的粘稠细胞外基质，其中大多数细菌处于休眠代谢状态。这些休眠细菌通常不受常规抗生素的影响。常规牙科线消毒剂很少能去除生物膜。一个更引人注目的解决方案是首先防止生物膜的形成。在第一阶段 STTR 项目中，我们将研究用于牙科水管内腔的硒基抗菌涂层的可行性。硒充当超氧自由基的催化发生器，从而杀死细菌。硒可以共价结合到水管内部，并可能永久抑制生物膜的形成。我们将开发聚氨酯牙科管的涂层方法，并测试管样品的涂层连续性和稳定性。我们还将确定涂层密度和抗菌功效之间的关系。最后，我们将评估涂层管在使用铜绿假单胞菌的流动池实验中的功效。