INACTIVATION OF BACTERIAL PATHOGENS--S AUREUS

细菌性病原体--金黄色葡萄球菌的灭活

• 批准号: 6285799
• 负责人: Ted W Reid
• 金额: $ 2.6万
• 依托单位: SELENIUM TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-03 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6285799
• 关键词: Staphylococcus aureus; antiinfective agents; bacterial virus; biotechnology; peptide library; peptides; selenium; superoxides; technology /technique development

This grant proposes to produce selenium labelled peptides and phage (bacterial viruses) that can selectively bind to the surface of pathogenic bacteria and inactivate them through the generation of superoxide radicals on their surface. For the purposes of this grant we will develop these labelled seleno- peptides and phage (from display libraries) against the bacteria Staphylococcus aureus. This proposal is based upon technology that has been found to be extremely selective and potent against pathogens. These seleno-peptides and selenium labelled phage should provide a safe means for instant protection from Staphylococcus aureus before it can cause damage. In addition, this approach utilizes a technology for which bacteria should not be able to become resistant since the superoxide radical killing of bacteria is lymphocytic mechanism that is used by the macrophage. The present grant proposal will: 1) select specific peptides and phage that bind selectively to the surface of Staphylococcus aureus; 2) increase the affinity and specificity of these specific peptides and phage by computer assisted evolution; 3) synthesize selenium labelled peptides and phage; and 4) test the ability of these selenium labelled peptides and phage to inactivate Staphylococcus aureus in vitro and in vivo.

这项资助计划生产硒标记的肽和噬菌体（细菌病毒），这些肽和噬菌体可以选择性地结合到致病细菌的表面，并通过在其表面产生超氧化物自由基来抑制它们。 为了这项资助的目的，我们将开发这些标记的硒肽和噬菌体（来自展示文库），以对抗金黄色葡萄球菌。 这一建议是基于一种对病原体具有极强选择性和效力的技术。这些硒肽和硒标记的噬菌体应该提供一种安全的手段，用于在金黄色葡萄球菌造成损害之前立即保护金黄色葡萄球菌。 此外，这种方法利用了一种细菌不应该产生抗性的技术，因为超氧化物自由基杀死细菌是巨噬细胞使用的淋巴细胞机制。本资助计划将：1）选择选择性结合金黄色葡萄球菌表面的特异性肽和噬菌体; 2）通过计算机辅助进化增加这些特异性肽和噬菌体的亲和力和特异性; 3）合成硒标记的肽和噬菌体;和4）测试这些硒标记的肽和噬菌体体外和体内杀灭金黄色葡萄球菌的能力。