Cell-specific transcriptional responses to environmental stress in Arabidopsis

拟南芥对环境胁迫的细胞特异性转录反应

• 批准号: 7221038
• 负责人: ANJALI S IYER-PASCUZZI
• 金额: $ 4.48万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7221038
• 关键词: Affect; Aluminum; Animals; Arabidopsis; Cell Line; Cell Proliferation; Cells; Complex; Condition; Development; Developmental Process; Environment; Gene Expression; Genes; Genetic Transcription; Goals; Growth; Growth and Development function; Histocompatibility Testing; Life; Maps; Methods; Microarray Analysis; Organ; Organism; Pattern; Plant Roots; Plants; Platelet Factor 4; Process; Protocols documentation; Resolution; Role; Shapes; Signal Transduction; Soil; Sorting - Cell Movement; Stimulus; Stress; Systems Biology; Tissues; Toxic effect; Translating; Variant; base; cell type; intracellular protein transport; mutant; plant growth/development; protein localization location; response; transcription factor

DESCRIPTION (provided by applicant): Environmental stress shapes plant development in part by affecting transcription factor (TF) networks. Comprehending these networks and how they respond to external stimuli is key to understanding both animal and plant development. Our long-term goals are 1) to understand how environmental signals are perceived and translated into developmental changes in the plant root, 2) to develop a profile of the root transcription network under various environmental stresses, and 3) to use this information in generating a systems biology approach towards understanding plant development and to look for broad commonalities between plant and animal transcription networks. We will focus on aluminum (Al) toxicity to study the relationship between environmental stress, root development, and transcription networks. Toxic levels of Al inhibit growth of the primary root, and are often found in acidic soils. Since TFs are essential to plant developmental processes, which are in turn affected by the environment, their expression should change in response to external stress. Thus, we hypothesize that Al inhibits root elongation by affecting the expression and/or protein localization of transcription factors involved in cell proliferation and elongation, two of the major processes in organ growth. Our lab has developed a protocol based on sorting fluorescently marked cell types to examine global gene expression at cell-specific resolution, and used it to develop an Arabidopsis root expression map under unstressed conditions. We will apply this method to understand TF responses to Al stress, and how such responses result in the inhibition of organ growth and development in Arabidopsis. Our specific aims are to: 1. Identify morphological changes in 6 different root cell types in response to Al with fluorescently marked cell lines. Although whole root effects of Al have been studied, effects of Al on specific root cell types are not well known. 2. Identify the set of genes affected by Al stress in the whole Arabidopsis root with microarray analysis. To identify potential tissue types most strongly affected by the stress, we will cross reference genes identified here with our root expression map created under unstressed conditions. 3. Perform detailed cell-specific microarray analysis on tissue types most affected by Al and identify the most altered TFs. 4. Clarify the role of selected TFs in Al toxicity and root growth by mutant characterization. 5. Examine selected TF expression in Arabidopsis accessions with different sensitivities to Al. Though plant and animal developmental processes are clearly different, the same basic problems had to be solved to result in a successful multicellular organism. The complex interactions through which life develops are thus likely to follow broadly parallel patterns.

描述（由申请人提供）：环境胁迫部分通过影响转录因子（TF）网络来影响植物发育。理解这些网络以及它们如何对外部刺激作出反应是理解动物和植物发育的关键。我们的长期目标是1)了解环境信号如何被感知并转化为植物根的发育变化，2)发展各种环境胁迫下的根转录网络概况，以及3)利用这些信息生成系统生物学方法来理解植物发育，并寻找植物和动物转录网络之间的广泛共性。我们将重点研究铝（Al）毒性，以研究环境胁迫、根发育和转录网络之间的关系。有毒水平的铝会抑制主根的生长，并且经常在酸性土壤中发现。由于TFs对植物的发育过程至关重要，而植物的发育过程又受环境的影响，因此它们的表达应随着外界胁迫的变化而变化。因此，我们假设Al通过影响参与细胞增殖和伸长的转录因子的表达和/或蛋白质定位来抑制根伸长，细胞增殖和伸长是器官生长的两个主要过程。我们的实验室开发了一种基于荧光标记细胞类型分选的方案，以细胞特异性分辨率检测全球基因表达，并使用它来开发非胁迫条件下的拟南芥根表达图谱。我们将运用这种方法来了解TF对Al胁迫的反应，以及这种反应如何导致拟南芥器官生长发育的抑制。我们的具体目标是：1。用荧光标记细胞系鉴定6种不同根细胞类型对铝的形态学变化。虽然已经研究了铝的全根效应，但铝对特定根细胞类型的影响尚不清楚。2. 利用微阵列分析鉴定拟南芥根系受铝胁迫影响的一组基因。为了确定受胁迫影响最大的潜在组织类型，我们将在这里鉴定的基因与我们在非胁迫条件下创建的根表达图进行交叉对照。3. 对受Al影响最大的组织类型进行详细的细胞特异性微阵列分析，并确定最改变的tf。4. 通过突变体表征阐明选定的tf在铝毒性和根生长中的作用。5. 研究对Al具有不同敏感性的拟南芥中选定的TF表达。尽管植物和动物的发育过程明显不同，但要成功培育出多细胞生物，必须解决相同的基本问题。因此，生命赖以发展的复杂的相互作用很可能遵循大致相似的模式。