A Water-Window Soft X-Ray Microscope for Small Laboratories

适用于小型实验室的水窗软 X 射线显微镜

• 批准号: 7269712
• 负责人: Stephen Horne
• 金额: $ 37.34万
• 依托单位: ENERGETIQ TECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7269712
• 关键词: 3-Dimensional; Biological; Cell physiology; Cells; Cellular Structures; Collection; Coupled; Data; Development; Diagnosis; Dimensions; Disadvantaged; Disease; Electron Microscopy; Freezing; Goals; Image; Imaging Techniques; Investigation; Laboratories; Lead; Light; Lighting; Low Dose Radiation; Measures; Microscope; Microscopy; Optics; Pattern; Phase; Photons; Plasma; Positioning Attribute; Preparation; Range; Research; Resolution; Roentgen Rays; Sampling; Shapes; Solid; Source; Specimen; Subcellular structure; Synchrotrons; System; Techniques; Technology; Testing; Thick; Time; Water; Work; base; cost; day; design; drug discovery; innovation; prototype; size; tool

DESCRIPTION (provided by applicant): Soft x-ray microscopy shows enormous promise as a technique for imaging cellular structures at resolutions well beyond what can be achieved in optical microscopes, and with much simpler sample preparation than is required for electron microscopy. In addition, the lower radiation dose required (compared to electron microscopy) allows tomographic investigation of subcellular structures in three dimensions. Existing synchrotron based microscopes have shown the potential of the soft x-ray microscope as a research tool, but they have the disadvantage of being tied to a massive light source at only a few national laboratories. A suitably bright, compact, low cost light source is the enabling innovation needed to realize a commercial biological soft x-ray microscope. Energetiq has developed a unique light source technology using an electrodeless, inductively coupled plasma to produce light in the soft x-ray range. The Energetiq light source can be used to generate light needed to illuminate and image biological specimens in a soft x-ray microscope. In Phase I Energetiq explored the capability of the light source in the "water window" range of wavelengths and determined that an x- ray microscope based on the light source is feasible. A "proof of principle" microscope will be constructed in Phase II. Biologists have a need to image whole biological cells both at high resolution and in their hydrated state. Currently available microscopes satisfy one of these requirements but not both. Soft x-ray microscopy in the so-called 'water window' allows resolution to 20nm for fully hydrated cell samples, and can produce 2-D and 3-D tomographic images. The ability to resolve sub cellular structures in hydrated cells, and to create those images in minutes rather than days, could lead to more rapid advances in drug discovery, disease diagnosis, disease treatment and better understanding of the fundamental cellular processes.

描述（由申请人提供）：软X射线显微镜显示出巨大的前景，作为一种技术，用于成像细胞结构的分辨率远远超过可以在光学显微镜中实现的，并且具有比电子显微镜所需的简单得多的样品制备。此外，所需的较低辐射剂量（与电子显微镜相比）允许三维亚细胞结构的断层扫描研究。现有的基于同步加速器的显微镜已经显示出软X射线显微镜作为研究工具的潜力，但它们的缺点是仅在少数国家实验室中被绑定到大规模光源。一个合适的明亮，紧凑，低成本的光源是实现商业生物软X射线显微镜所需的使能创新。Energetiq开发了一种独特的光源技术，使用无电极感应耦合等离子体产生软X射线范围内的光。Energetiq光源可用于产生在软X射线显微镜中照明和成像生物标本所需的光。在第一阶段，Energetiq探索了光源在“水窗”波长范围内的能力，并确定基于该光源的X射线显微镜是可行的。第二阶段将建造一个“原理证明”显微镜。生物学家需要以高分辨率和水合状态对整个生物细胞进行成像。目前可用的显微镜满足这些要求之一，但不是两个。所谓的“水窗”中的软X射线显微镜允许完全水合细胞样品的分辨率达到20 nm，并且可以产生2-D和3-D断层图像。在水合细胞中解析亚细胞结构的能力，以及在几分钟而不是几天内创建这些图像的能力，可能会导致药物发现，疾病诊断，疾病治疗和更好地理解基本细胞过程的更快进展。