A High Brightness Light Source for UV-VIS Spectroscopic Imaging

用于紫外-可见光谱成像的高亮度光源

• 批准号: 7799627
• 负责人: Stephen Horne
• 金额: $ 19.81万
• 依托单位: ENERGETIQ TECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-24 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7799627
• 关键词: 3T3 Cells; Biological; Biological Assay; Boston; Cells; Data; Engineering; Family; Filtration; Gases; Generations; Goals; Guidelines; Image; Image Analysis; Injection of therapeutic agent; Lasers; Light; Light Microscope; Maps; Measurement; Mechanics; Medical Research; Microscope; Microscopic; Microscopy; Modeling; Modification; Molecular; Optics; Oranges; Output; Performance; Phase; Plasma; Principal Investigator; Production; Productivity; Property; Relative (related person); Reporting; Resolution; Sampling; Semiconductors; Source; Spectrophotometry; Staining method; Stains; System; Techniques; Technology; Testing; Time; TimeLine; Universities; Variant; Work; Writing; Xenon; base; biological systems; commercialization; cost; design; drug development; fluorescence imaging; imaging modality; improved; instrument; metrology; operation; pressure; programs; prototype; public health relevance; spectroscopic imaging; success; tool; ultraviolet

DESCRIPTION (provided by applicant): Energetiq has developed an ultra-high-brightness light source for semiconductor metrology applications. This same basic technology could have broad application in biological imaging, where today's instruments are limited in optical brightness, particularly in the UV spectral region. The goal of this proposed program, "A High Brightness Light Source for UV-VIS Spectroscopic Imaging" is to develop a new family of light sources optimized for quantitative UV-VIS spectroscopic imaging. Our Phase I program will (i) prototype a laser-driven plasma source optimized for microscopy; (ii) design a wavelength-filtered condenser system matched to a broad-band biological microscope; then (iii) begin to demonstrate the advantages of this source in imaging of biological cells. PUBLIC HEALTH RELEVANCE: Broad-band optical microscopy is a key tool for a wide range of biological studies. However today's instruments are limited, both generally in optical brightness and in particular in the deep UV region, where high-resolution imaging spectrophotometry shows the most potential value. The goal of this proposed program, "A High Brightness Light Source for UV-VIS Spectroscopic Imaging" is to develop a new family of light sources optimized for quantitative UV-VIS spectroscopic imaging. Improved throughput and information content for biological assay is central to the productivity both of medical research and drug development.

描述（由申请人提供）：Energetiq开发了一种用于半导体计量应用的超高亮度光源。同样的基本技术可以在生物成像中有广泛的应用，其中今天的仪器在光学亮度方面受到限制，特别是在紫外光谱区域。该计划的目标是“用于UV-VIS光谱成像的高亮度光源”，旨在开发一种新的光源系列，用于定量UV-VIS光谱成像。我们的第一阶段计划将（一）原型的激光驱动等离子体源优化显微镜;（二）设计一个波长过滤的聚光系统匹配的宽带生物显微镜;然后（三）开始，以证明这种来源的生物细胞成像的优势。 公共卫生相关性：宽带光学显微镜是广泛生物学研究的关键工具。然而，今天的仪器是有限的，无论是一般在光学亮度，特别是在深紫外区，高分辨率成像分光光度法显示出最有潜力的价值。该计划的目标是“用于UV-VIS光谱成像的高亮度光源”，旨在开发一种新的光源系列，用于定量UV-VIS光谱成像。提高生物测定的通量和信息含量对于医学研究和药物开发的生产力都是至关重要的。