The Role of SDF-1 Cerebral Repair Following Stroke

描述（由申请人提供）：中风是国家的第三大死因和长期残疾的主要原因。初步数据显示，在大脑修复过程中，基质细胞衍生因子-1（SDF-1）在小鼠大脑中上调。SDF-1被认为对许多细胞类型具有趋化性，并促进血管生成。我假设SDF-1的上调是由于缺氧引起的，并通过诱导新泡形成和将骨髓来源的细胞归巢到损伤部位来介导缺血后大脑的修复机制，从而导致功能恢复的总体增加。为了验证我的假设，我打算：（1）使用新的GFP嵌合小鼠中风模型确定SDF-1对脑的新血管形成的贡献，并确定缺血后功能恢复的增加，（2）确定SDF-1是否通过上调血管生成或通过上调血管发生在体外诱导新血管形成，（3）探讨SDF-1在缺氧反应中的上调是否受HIF-1的调节。了解中风后的内源性修复机制可能会导致新的治疗方法来增强自然修复过程，这可能会降低死亡率并限制中风患者的长期残疾。

DESCRIPTION (PROVIDED BY APPLICANT): Stroke is the nation's third leading cause of death and the leading cause of long-term disability. Preliminary data show that during cerebral repair, Stromal Cell-Derived Factor-1 (SDF-1) is upregulated in the brain of mice. SDF-1 is thought to be chemotactic for many cell types, and to promote angiogenesis. I hypothesize that the upregulation of SDF-1 is due to hypoxia and mediates the repair mechanisms of the brain following ischemia by inducing neovsacularization and by homing bone marrow derived cells to the site of injury leading to an overall increase in functional recovery. To test my hypothesis, I plan to: (1) determine the contribution of SDF-1 to neovascularization of the brain and determine the increase in functional recovery post-ischemia using a novel GFP chimeric mouse stroke model, (2) determine whether SDF-1 induces neovascularization in vitro by upregulating angiogenesis or by upregulating vasculogenesis, (3) investigate whether the upregulation of SDF-1 in response to hypoxia is under HIF-1 regulation in vitro. Understanding the endogenous repair mechanisms following stroke could lead to novel treatments to enhance the natural repair processes, which could decrease mortality and limit long-term disability seen in stroke patients.