Laser Activated Ophthalmic Adhesive

激光激活眼科粘合剂

• 批准号: 7472807
• 负责人: BARBARA Ann SOLTZ
• 金额: $ 7.15万
• 依托单位: CONVERSION ENERGY ENTERPRISES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7472807
• 关键词: Adhesions; Adhesives; Anastomosis - action; Animal Experiments; Animal Model; Animals; Area; Biocompatible Materials; Biodegradation; Blindness; Blood Vessels; Blunt Trauma; Bulla; Cataract; Cataract Extraction; Chemistry; Clinical; Clinical Trials; Closure; Collagen; Conjunctival Pterygium; Cornea; Corneal Injury; Corneal Ulcer; Cross-Linking Reagents; Cyanoacrylates; Data; Development; Doctor of Philosophy; Drug Formulations; End Point; Ensure; Excision; Extracapsular; Eye; Eye diseases; Film; Foundations; Funding; Glaucoma; Glues; Goals; Grant; Human; Impairment; In Situ; In Vitro; Institutes; Investigation; Keratoplasty; Laboratory Research; Laceration; Lamellar Keratoplasty; Laser In Situ Keratomileusis; Lasers; Lead; Measures; Mechanics; Medical Electronics; Membrane; Methods; Modeling; National Eye Institute; Object Attachment; Operative Surgical Procedures; Outcome; Penetrating Keratoplasty; Phase; Phase I Clinical Trials; Phase II Clinical Trials; Postoperative Period; Preparation; Principal Investigator; Procedures; Process; Property; Proteins; Psychological reinforcement; Radial Keratotomy; Rate; Reaction; Reagent; Research; Research Personnel; Retinal; Risk; Rupture; Sampling; Secure; Site; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Solutions; Standards of Weights and Measures; Sterility; Sterilization for infection control; Strabismus; Surgical Flaps; Surgical Models; Surgical incisions; Surgical sutures; Survivors; System; Techniques; Technology; Temperature; Tensile Strength; Testing; Thick; Tissue Adhesives; Tissues; Trauma; United States Dept. of Health and Human Services; United States Food and Drug Administration; United States National Institutes of Health; Vision; Welding; Work; anterior chamber; articular cartilage; base; biomaterial compatibility; cell injury; commercialization; conjunctiva; corneal surgery; design; experience; improved; in vitro Model; in vivo; innovation; pre-clinical; pressure; protein crosslink; reconstruction; repaired; research study; response; seal; sensor; skills; tissue glueing; tissue soldering; tissue welding; wound

Project Summary The significance of this project is the development of a laser assisted tissue welding technique to close corneal wounds, provide a foundation for innovative treatment of eye disease and demonstrate the unique properties of a laser cured collagen adhesive to repair ophthalmic tissue with superior strength and stability. Millions of ophthalmic procedures are performed annually, most incorporating sutures to close and seal wounds. Yet this method can cause severe complications, can weaken the integrity of the eye and may pose a risk for ocular rupture in the case of blunt trauma. Other techniques to close wounds, including the use of tissue glues or grafting, can be even more problematic. Phase I studies were conducted to measure the biostability of solder formulations, evaluate tissue bonding strategies and compare wound stability as a function of intrachamber pressure in four in vitro models of clinical corneal surgery: radial keratotomy, LASIK flaps, penetrating keratoplasty and cataract excision. The results confirmed that the laser solder approach is superior to standard sutures with no evidence of tissue shrinkage. The strength of the soldered repair was dependent upon reaching a precise temperature set by choice of laser, solder composition and film thickness. Solder biostability is increased by reaction with a standard crosslinking reagent. The specific aim for Phase II is to optimize solder processing, laser techniques and surgical outcome in survivor animal surgical models. Task descriptions include preparation of sterile solder under stringent process controls, design and assembly of a compact preclinical laser systems, optimization of a laser handpiece and perform ex vivo and in vivo experiments on a lapine model. Surgical endpoints will include the quality of the wound seal, the extent, if any, of collateral thermal tissue damage, solder stability and biocompatibility.

项目摘要 本课题的意义在于发展一种激光辅助组织焊接技术来闭合角膜 伤口，为创新治疗眼病提供了基础，并展示了 一种激光固化的胶原粘合剂，用于修复具有上级强度和稳定性的眼组织。数百万 眼科手术每年进行一次，大多数都使用缝合线来闭合和密封伤口。然而这 这种方法可能会导致严重的并发症，可能会削弱眼睛的完整性，并可能对眼睛造成风险。 钝伤导致的破裂闭合伤口的其他技术，包括使用组织胶或 嫁接，可能会更麻烦。进行I期研究以测量焊料的生物稳定性 制剂，评估组织粘合策略并比较伤口稳定性作为内室的函数 在四种临床角膜手术的体外模型中的压力：放射状角膜切开术、LASIK皮瓣、穿透性角膜移植术、角膜移植术、角膜移植术。 角膜移植术和白内障切除术。结果证实，激光焊接方法优于上级 标准缝线，无组织收缩迹象。焊接修复的强度取决于 在达到通过选择激光、焊料成分和膜厚度而设定的精确温度时。焊料 通过与标准交联剂反应来提高生物稳定性。第二阶段的具体目标是 在存活动物手术模型中优化焊接处理、激光技术和手术结果。任务 描述包括在严格的过程控制下制备无菌焊料， 紧凑型临床前激光系统，优化激光手柄，并在体外和体内执行 在兔模型上进行的实验。手术终点将包括伤口密封的质量、程度（如果有）， 附带热组织损伤、焊接稳定性和生物相容性。