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The Primate Embryo Gene Expression Resource

灵长类动物胚胎基因表达资源

基本信息

批准号：
7233654
负责人：
Keith E Latham
金额：
$ 46.01万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-09-30 至 2008-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7233654
关键词：
Animal Husbandry Animal Model Animals Area Basic Science Biological Markers Biological Models Cattle Cell Therapy Cells Clinical Communities Compatible Data Databases Development Disease Dot Immunoblotting Embryo Embryology Embryonic Development Emerging Technologies Evaluation Event Fertilization Fertilization in Vitro Funding Gene Expression Genes Health Hormonal Human In Vitro Inner Cell Mass Knowledge Libraries Macaca mulatta Messenger RNA Methods Modeling Molecular Molecular Biology Mus National Center for Research Resources Numbers Oocytes Operative Surgical Procedures Oryctolagus cuniculus Papio Pattern Population Preimplantation Diagnosis Primates Procedures Production Proteins Protocols documentation Purpose Rate Reproduction Reproductive Medicine Research Research Personnel Resources Sampling Scientist Screening procedure Staging Stem cells System Techniques Testing base blastocyst cDNA Library clinical effect cost gene discovery gene function hatching in vivo interest mammalian embryology mature animal natural Blastocyst Implantation nonhuman primate novel oocyte maturation programs research study stem cell therapy subtraction hybridization success tool

项目摘要

DESCRIPTION (provided by applicant): The Primate Embryo Gene Expression Resource permits the rapid and cost-effective study of gene expression in primate oocytes and preimplantation embryos using the rhesus monkey as the primary model organism. PREGER contains over 160 informative samples representing GV-stage oocytes, MIIstage oocytes, and embryos at the 1-cell through hatched blastocyst stages, obtained using different combinations of in vitro or in vivo maturation, fertilization, and culture. This extensive and valuable sample set permits analyses of gene expression related to understanding basic primate embryology, as well as potential effects of clinical procedures on embryo quality using a primate model. Dot blots for expression analysis, cDNA libraries, probes, and other tools in PREGER can be distributed to other scientists, providing unprecedented opportunities for molecular studies in primate embryos. The libraries can be employed for a myriad of other purposes, such as library screening and library subtraction. We propose to maintain the resource, continue to distribute these materials and molecular tools to the scientific community, maintain the PREGER website, extend the resource to encompass samples from baboon in order that the generality of observations among primate species may be evaluated, and incorporate samples of human and rhesus ES lines and rhesus inner cell mass cells for evaluating the developmental potency of human ES lines. We also we propose to apply a novel method for suppression subtraction hybridization that we have developed to be compatible with our amplified libraries, in order to identify novel developmentally regulated genes that are relevant to early primate embryogenesis and that will assist improvements in assisted reproduction methods. This will facilitate basic research, and may support novel clinical approaches, e.g., in the evaluation of embryo quality. The proposed activities will at once maintain and expand the resource, produce new and fundamental basic information about primate embryos, and produce novel molecular approaches that may be useful in clinical practice.

描述（由申请人提供）：