THE PRIMATE EMBRYO GENE EXPRESSION RESOURCE

灵长类胚胎基因表达资源

• 批准号: 8172545
• 负责人: Keith E Latham
• 金额: $ 7.6万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172545
• 关键词: Animal Model; Basic Science; Cells; Clinical; Computer Retrieval of Information on Scientific Projects Database; Databases; Dot Immunoblotting; Embryo; Embryology; Evaluation; Fertilization; Funding; Gene Expression; Grant; In Vitro; Institution; Libraries; Macaca mulatta; Modeling; Molecular; Oocytes; Ovarian; Primates; Procedures; Research; Research Personnel; Resources; Sampling; Scientist; Source; Staging; Stem cells; Training; United States National Institutes of Health; blastocyst; cDNA Library; clinical effect; clinical practice; cost effective; hatching; in vivo; novel; tool; web site

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Primate Embryo Gene Expression Resource (PREGER) permits the rapid and cost-effective study of gene expression in primate oocytes and preimplantation embryos using the rhesus monkey as the primary model organism. PREGER contains over 200 informative samples representing GV-stage oocytes, MIIstage oocytes, embryos at the 1-cell through hatched blastocyst stages, obtained using different combinations of in vitro or in vivo maturation, fertilization, and culture. The resource also contains stem cells and ovarian cells. This extensive and valuable sample set permits analyses of gene expression related to understanding basic primate embryology, as well as potential effects of clinical procedures on embryo quality using a primate model. Dot blots for expression analysis, cDNA libraries, probes, and other tools in PREGER can be distributed to other scientists, providing unprecedented opportunities for molecular studies in primate embryos. The libraries can be employed for a myriad of other purposes. The resource also includes a web site and online database. Most recently, the resource has incorporated an annual molecular embryology training course. The resource will facilitate basic research, and may support novel clinical approaches, e.g., in the evaluation of embryo quality. The proposed activities will at once maintain and expand the resource, produce new and fundamental basic information about primate embryos, and produce novel molecular approaches that may be useful in clinical practice.

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