THE PRIMATE EMBRYO GENE EXPRESSION RESOURCE

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 灵长类胚胎基因表达资源(PREGER)能够以恒河猴为主要模式生物，对灵长类动物卵母细胞和植入前胚胎中的基因表达进行快速和经济有效的研究。PREGER包含200多个信息样本，代表GV阶段的卵母细胞、MII阶段的卵母细胞、1-细胞到孵化囊胚阶段的胚胎，这些胚胎是通过体外或体内成熟、受精和培养的不同组合获得的。该资源还包括干细胞和卵巢细胞。这一广泛而有价值的样本集允许使用灵长类动物模型分析与理解基本灵长类胚胎学相关的基因表达，以及临床操作对胚胎质量的潜在影响。PREGER中用于表达分析的斑点杂交、cDNA文库、探针和其他工具可以分发给其他科学家，为灵长类胚胎的分子研究提供了前所未有的机会。这些库可以用于无数其他目的。该资源还包括一个网站和在线数据库。最近，该资源纳入了一年一度的分子胚胎学培训课程。该资源将促进基础研究，并可能支持新的临床方法，例如在胚胎质量评估方面。拟议的活动将立即维持和扩大资源，产生关于灵长类胚胎的新的基本信息，并产生可能在临床实践中有用的新的分子方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Primate Embryo Gene Expression Resource (PREGER) permits the rapid and cost-effective study of gene expression in primate oocytes and preimplantation embryos using the rhesus monkey as the primary model organism. PREGER contains over 200 informative samples representing GV-stage oocytes, MIIstage oocytes, embryos at the 1-cell through hatched blastocyst stages, obtained using different combinations of in vitro or in vivo maturation, fertilization, and culture. The resource also contains stem cells and ovarian cells. This extensive and valuable sample set permits analyses of gene expression related to understanding basic primate embryology, as well as potential effects of clinical procedures on embryo quality using a primate model. Dot blots for expression analysis, cDNA libraries, probes, and other tools in PREGER can be distributed to other scientists, providing unprecedented opportunities for molecular studies in primate embryos. The libraries can be employed for a myriad of other purposes. The resource also includes a web site and online database. Most recently, the resource has incorporated an annual molecular embryology training course. The resource will facilitate basic research, and may support novel clinical approaches, e.g., in the evaluation of embryo quality. The proposed activities will at once maintain and expand the resource, produce new and fundamental basic information about primate embryos, and produce novel molecular approaches that may be useful in clinical practice.