THE PRIMATE EMBRYO GENE EXPRESSION RESOURCE

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 灵长类动物胚胎基因表达资源允许使用恒河猴作为主要模型生物体，对灵长类动物卵母细胞和植入前胚胎中的基因表达进行快速且经济有效的研究。 PREGER 包含 200 多个信息样本，代表 GV 期卵母细胞、MII 期卵母细胞、1 细胞胚胎到孵化囊胚期，这些样本是通过体外或体内成熟、受精和培养的不同组合获得的。 该资源还包含干细胞和卵巢细胞。 这个广泛且有价值的样本集允许分析与了解基本灵长类动物胚胎学相关的基因表达，以及使用灵长类动物模型临床程序对胚胎质量的潜在影响。 PREGER 中用于表达分析的点印迹、cDNA 文库、探针和其他工具可以分发给其他科学家，为灵长类动物胚胎的分子研究提供前所未有的机会。这些库可用于多种其他目的。该资源还包括网站和在线数据库。 最近，该资源纳入了年度分子胚胎学培训课程。 该资源将促进基础研究，并可能支持新的临床方法，例如胚胎质量的评估。拟议的活动将立即维护和扩展资源，产生有关灵长类动物胚胎的新的基本信息，并产生可能在临床实践中有用的新分子方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Primate Embryo Gene Expression Resource permits the rapid and cost-effective study of gene expression in primate oocytes and preimplantation embryos using the rhesus monkey as the primary model organism. PREGER contains over 200 informative samples representing GV-stage oocytes, MIIstage oocytes, embryos at the 1-cell through hatched blastocyst stages, obtained using different combinations of in vitro or in vivo maturation, fertilization, and culture. The resource also contains stem cells and ovarian cells. This extensive and valuable sample set permits analyses of gene expression related to understanding basic primate embryology, as well as potential effects of clinical procedures on embryo quality using a primate model. Dot blots for expression analysis, cDNA libraries, probes, and other tools in PREGER can be distributed to other scientists, providing unprecedented opportunities for molecular studies in primate embryos. The libraries can be employed for a myriad of other purposes. The resource also includes a web site and online database. Most recently, the resource has incorporated an annual molecular embryology training course. The resource will facilitate basic research, and may support novel clinical approaches, e.g., in the evaluation of embryo quality. The proposed activities will at once maintain and expand the resource, produce new and fundamental basic information about primate embryos, and produce novel molecular approaches that may be useful in clinical practice.