800 MHZ NMR SPECTROMETER FOR HIGH-END NMR STRUCTURAL STUDIES: AIDS

用于高端 NMR 结构研究的 800 MHZ NMR 波谱仪：艾滋病

• 批准号: 7335332
• 负责人: JOHN Hackett BUSHWELLER
• 金额: $ 5万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335332
• 关键词: AIDS; detergents; magnetism; membrane proteins; noise; performance; solubility; solutions; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. Regarding AIDS DESCRIPTION (provided by applicant): Eight faculty members at the University of Virginia are using high-resolution multi-dimensional NMR in their studies of biopolymers. These investigators are all well-funded with grants from the NIH and other sources. The use of NMR to elucidate biomolecular structure and function is an integral part of their NIH funded research. Due to the challenges of the systems being worked on by these investigators, including limitations in solubility and/or stability, lack of dispersion (spread), and a focus on large systems such as integral membrane proteins solubilized in detergents, this group has identified a distinct need for higher field NMR instrumentation on campus. To this end, we are requesting funding for an 18.8 T (800 MHz 1H) nuclear magnetic resonance (NMR) spectrometer to be sited at the University of Virginia. This spectrometer will be used for high-resolution multi- dimensional NMR experiments with labeled (15N, 13C and 15N, 13C, 2H) biopolymers in solution as well as in the presence of various alignment media. In order to accommodate the requirements of the installation site, the magnet will be actively shielded to minimize the effects of the stray field on neighboring laboratories. The spectrometer will be equipped with 4 radiofrequency (RF) channels with one dual fullband RF system capable of operating at the 1H frequency and lower and one dual broadband RF system capable of operating at the 15N to 31P frequency range. The spectrometer will also be equipped with a He-cooled triple resonance 1H-detection cryoprobe equipped with a single-axis pulsed-field gradient capability. This probe provides a dramatic improvement in the signal-to-noise which is essential for the majority of the user applications. The 800 MHz NMR spectrometer will provide dramatic enhancements in three critical performance criteria that are essential for the research efforts of our user group: 1) The 800 MHz NMR spectrometer provides a substantial increase in the signal-to-noise (S/N). 2) The 800 MHz NMR spectrometer provides a substantial increase in the dispersion (spread) of NMR signals. 3) The 800 MHz NMR spectrometer enhances the performance of TROSY-based experiments.

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。关于艾滋病的描述(由申请人提供)：弗吉尼亚大学的八名教职员工在他们的生物聚合物研究中使用高分辨率多维核磁共振。这些调查人员都有来自美国国立卫生研究院和其他来源的资金。使用核磁共振来阐明生物分子结构和功能是他们NIH资助的研究的组成部分。由于这些研究人员正在研究的系统面临的挑战，包括溶解度和/或稳定性的限制，缺乏分散(扩散)，以及专注于大型系统，如溶解在洗涤剂中的完整膜蛋白，该小组确定了在校园内对更高场核磁共振仪器的明显需求。为此，我们正在申请资金，在弗吉尼亚大学建造一台18.8T(800 MHz 1H)核磁共振(核磁共振)光谱仪。该光谱仪将用于标记(15N，13C和15N，13C，2H)生物聚合物在溶液中以及在各种排列介质中的高分辨率多维核磁共振实验。为了适应安装现场的要求，磁体将被主动屏蔽，以最大限度地减少杂散磁场对邻近实验室的影响。光谱仪将配备4个射频通道，其中一个可在1H频率及更低频率下工作的双全频射频系统，以及一个可在15N至31P频率范围内工作的双宽带射频系统。该光谱仪还将配备一个氦冷却三重共振1H探测低温探头，配备单轴脉冲场梯度能力。该探头极大地改善了信噪比，这对大多数用户应用来说是必不可少的。800 MHz核磁共振光谱仪将在对我们用户群的研究工作至关重要的三个关键性能标准方面提供显著增强：1)800 MHz核磁共振光谱仪可大幅提高信噪比(S/N)。2)800 MHz核磁共振谱仪大大增加了核磁共振信号的色散(扩展)。3)800 MHz核磁共振谱仪提高了基于TROSY的实验性能。