Biacore T100

描述（由申请人提供）：本提案中要求的仪器Biacore T100是一种用于测量表面等离子体共振（SPR）的自动化设备。Biacore仪器使用微流体系统测量SPR，该微流体系统提供定量分析能力，以详细研究两种分子之间结合的特异性、特定分子的浓度、蛋白质动力学和两种蛋白质之间的结合亲和力。实时定量数据的产生将有助于乔治敦大学的研究人员进一步了解蛋白质、肽和核酸相互作用的方式和原因。这些知识将用于推进基础和应用科学知识，重点关注癌症生物学和新型癌症疗法。Lombardi能够从NIH盈余中获得使用过的Biacore 1000，并在我们开始使用SPR实现我们的研究目标时生成初步数据。在乔治敦大学使用B1000使我们有机会获得仪器和SPR的经验和专业知识。Toretsky博士、Wellstein博士和Roy博士这三位主要用户已经使用B1000为他们的项目生成了关键数据。所有三位研究者都需要SPR测量来推进他们的项目，现在正在准备这些新数据的手稿。所有被列为主要用户的研究者都资助了需要增强SPR能力的项目。琼斯博士有一个建议，包括一个需要SPR的目标。Pestell博士和Rui博士都有多个已建立的项目，这些项目将从SPR中受益匪浅。下文各项目章节概述了这些效益。我们的科学需求已经超过了B1000的能力。这些需求包括增强检测小分子相互作用的能力，温度动力学，以及更快的吞吐量，以适应乔治敦隆巴迪癌症中心日益增长的兴趣和越来越多的用户。T100的这些增强功能将使研究许多类型生物过程的项目得以推进。

DESCRIPTION (provided by applicant): The instrument requested in this proposal, the Biacore T100, is an automated device to measure surface plasmon resonance (SPR). The Biacore instrument measures SPR using a microfluidic system that provides quantitative analysis capabilities to study, in detail, the specificity of the binding between two molecules, concentrations of specific molecules, protein kinetics, and binding affinity between two proteins. The generation of real-time quantitative data will help Georgetown University investigators further understand how and why proteins, peptides, and nucleic acids interact. This knowledge will be used to advance both basic and applied scientific knowledge with a significant focus on cancer biology and novel cancer therapies. Lombardi was able to obtain a used Biacore 1000 from NIH surplus and generate preliminary data as we begin to work towards achieving our research goals using SPR. Working with the B1000 at Georgetown University has given us an opportunity to gain experience and expertise with the instrument and SPR. Three of the major users, Drs. Toretsky, Wellstein, and Roy, have used the B1000 to generate key data for their projects. All three investigators required the SPR measurements to advance their projects and are now preparing manuscripts with this new data. All investigators listed as Major Users have funded projects that require enhanced SPR capabilities. Dr. Jones has a proposal and includes an aim that requires SPR. Drs. Pestell and Rui both have multiple established projects that will benefit considerably from SPR. These benefits are outlined in their project sections below. Our scientific needs have exceeded the capacity of the B1000. These needs include the enhanced ability of detecting smaller molecule interactions, temperature kinetics, and more rapid throughput to accommodate the growing interest and increased number of users at Georgetown's Lombardi Cancer Center. These enhanced capabilities of the T100 will allow projects investigating many types of biologic processes to advance.