Transgenic and Knockout Mouse Resource

转基因和基因敲除小鼠资源

• 批准号: 7438466
• 负责人: Ruby Yanru Chen-Tsai
• 金额: $ 3.46万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-04 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7438466
• 关键词: Accounting; Advanced Malignant Neoplasm; Animal Model; Biological; Cancer Center; Causations; Consult; Cost Savings; Derivation procedure; ES Cell Line; Embryo; Foundations; Future; Gene Targeting; Genes; Goals; Human; Journals; Knockout Mice; Malignant Neoplasms; Microinjections; Molecular; Mus; Mutate; Pathogenesis; Peer Review; Plants; Production; Publications; Rate; Reagent; Research; Research Personnel; Resource Sharing; Resources; Role; Scientific Advances and Accomplishments; Services; Stem Cell Research; Stem cells; System; Technology; Transgenic Organisms; anticancer research; blastocyst; cost; embryonic stem cell; in vivo; medical schools; member; mouse genome; mouse model; programs; somatic cell nuclear transfer; tool

The Transgenic and Knockout Mouse Shared Resource was established in 1996 at the School of Medicine. The overall objective of this Shared Resource is to provide genetically modified mouse models and supporting technologies to Stanford Cancer Center investigators at cost-effective rates. This Shared Resource provides the necessary scientific and technical foundation and physical plant to conduct transgenic, gene targeting and embryonic stem cell research. The Shared Resource serves an important role in advancing cancer research by providing a wide spectrum of mouse embryo and stem cell manipulation and microinjection services to investigators as a highly specialized technology. The objectives of the Shared Resource are to: ¿ Consult in all aspects of creating and characterizing genetically modified mice ¿ Produce transgenic and knockout mice by microinjection ¿ Manipulate mouse embryonic stem cells for gene targeting ¿ Provide molecular and biological reagents associated with production of genetically modified mice Substantial cost savings, efficiencies and scientific advances accrue from providing these services through a centralized facility. The Shared Resource has generated over 500 mouse models in the last 5 years, each with multiple independent lines. The Shared Resource's services were acknowledged in more than 50 publications in peer-reviewed journals. In fiscal year 2005, there were 192 service requests, with Cancer Center members accounting for 56% of overall use. Cancer Center members representing eight out of ten Programs utilized the Shared Resource. Future plans for the Shared Resource include implementation of somatic cell nuclear transfer and tetraploid blastocyst complementation services, and derivation of new ES cell lines.

转基因和基因敲除小鼠共享资源于1996年在麻省理工学院 医学。这个共享资源的总体目标是提供转基因小鼠 以符合成本效益的价格向斯坦福癌症中心的研究人员提供模型和支持技术。这 共享资源为实现以下目标提供了必要的科技基础和物理基础 进行转基因、基因打靶和胚胎干细胞研究。共享资源服务于 通过提供广泛的小鼠胚胎和干细胞在推进癌症研究中的重要作用 细胞操纵和显微注射作为一项高度专业化的技术为调查人员提供服务。这个 共享资源的目标是： ？在创造和鉴定转基因小鼠的各个方面提供咨询 ？通过显微注射产生转基因和基因敲除小鼠 操纵小鼠胚胎干细胞进行基因打靶 ？提供与转基因小鼠生产相关的分子和生物试剂 提供这些服务可节省大量成本、提高效率并促进科学进步 通过一个集中的设施。在过去的5年中，共享资源已经生成了500多个鼠标模型 几年，每一条都有多条独立的线路。共享资源的服务已在 在同行评议期刊上发表了50多篇文章。在2005财政年度，有192项服务请求， 癌症中心成员占总使用量的56%。代表癌症中心的成员 每十个程序中就有八个使用共享资源。共享资源的未来计划包括 实施体细胞核移植和四倍体囊胚补充服务，以及 新ES细胞系的建立。