Transgenic and Knockout Mouse Resource

转基因和基因敲除小鼠资源

• 批准号: 7623562
• 负责人: Ruby Yanru Chen-Tsai
• 金额: $ 4.2万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7623562
• 关键词: Accounting; Advanced Malignant Neoplasm; Animal Model; Biological; Cancer Center; Causations; Consult; Cost Savings; Derivation procedure; ES Cell Line; Embryo; Foundations; Future; Gene Targeting; Genes; Goals; Human; Journals; Knockout Mice; Malignant Neoplasms; Microinjections; Molecular; Mus; Mutate; Pathogenesis; Peer Review; Plants; Production; Publications; Rate; Reagent; Research; Research Personnel; Resource Sharing; Resources; Role; Scientific Advances and Accomplishments; Services; Stem Cell Research; Stem cells; System; Technology; Transgenic Organisms; anticancer research; blastocyst; cost; embryonic stem cell; in vivo; medical schools; member; mouse genome; mouse model; programs; somatic cell nuclear transfer; tool

The Transgenic and Knockout Mouse Shared Resource was established in 1996 at the School of Medicine. The overall objective of this Shared Resource is to provide genetically modified mouse models and supporting technologies to Stanford Cancer Center investigators at cost-effective rates. This Shared Resource provides the necessary scientific and technical foundation and physical plant to conduct transgenic, gene targeting and embryonic stem cell research. The Shared Resource serves an important role in advancing cancer research by providing a wide spectrum of mouse embryo and stem cell manipulation and microinjection services to investigators as a highly specialized technology. The objectives of the Shared Resource are to: ¿ Consult in all aspects of creating and characterizing genetically modified mice ¿ Produce transgenic and knockout mice by microinjection ¿ Manipulate mouse embryonic stem cells for gene targeting ¿ Provide molecular and biological reagents associated with production of genetically modified mice Substantial cost savings, efficiencies and scientific advances accrue from providing these services through a centralized facility. The Shared Resource has generated over 500 mouse models in the last 5 years, each with multiple independent lines. The Shared Resource's services were acknowledged in more than 50 publications in peer-reviewed journals. In fiscal year 2005, there were 192 service requests, with Cancer Center members accounting for 56% of overall use. Cancer Center members representing eight out of ten Programs utilized the Shared Resource. Future plans for the Shared Resource include implementation of somatic cell nuclear transfer and tetraploid blastocyst complementation services, and derivation of new ES cell lines.

转基因和基因敲除小鼠共享资源于 1996 年在该学院建立 药品。该共享资源的总体目标是提供转基因小鼠 以具有成本效益的价格向斯坦福癌症中心研究人员提供模型和支持技术。这 共享资源提供了必要的科学技术基础和物理设施 进行转基因、基因靶向和胚胎干细胞研究。共享资源服务于 通过提供广泛的小鼠胚胎和干细胞，在推进癌症研究方面发挥重要作用 细胞操作和显微注射服务为研究人员提供了高度专业化的技术。这 共享资源的目标是： ¿ 就转基因小鼠的创建和表征的各个方面提供咨询 ¿ 通过显微注射产生转基因和基因敲除小鼠 ¿ 操纵小鼠胚胎干细胞进行基因靶向 ¿ 提供与转基因小鼠生产相关的分子和生物试剂 提供这些服务可节省大量成本、提高效率并取得科学进步 通过集中设施。共享资源在过去 5 年内已生成超过 500 个鼠标模型 年，每个都有多个独立的线路。共享资源的服务得到了认可 在同行评审期刊上发表了 50 多篇论文。 2005 财年，共有 192 项服务请求， 其中癌症中心会员占总体使用量的 56%。癌症中心成员代表 十分之八的程序利用了共享资源。共享资源的未来计划包括 实施体细胞核移植和四倍体囊胚互补服务，以及 新ES细胞系的衍生。