Animal Tumor Models

动物肿瘤模型共享资源在转基因等三大领域提供服务 以及基因敲除小鼠、临床前肿瘤学和动物组织病理学服务。转基因和 基因敲除小鼠生产服务于1996年在医学院建立，总体上 向斯坦福大学提供转基因小鼠模型和支持技术的目标 以符合成本效益的价格进行调查。卫生部兽医服务中心(VSC) 比较医学运行共享资源的动物组织病理学服务部分。主修 服务包括组织学肿瘤分析、免疫组织化学和病理解释。这个 临床前肿瘤学服务是动物肿瘤模型共享资源的新成员。这些 由癌症中心资助和建立的服务，以支持开发和测试 新化合物和更好地满足癌症中心成员对临床前日益增长的需求 动物模型和治疗学。共享资源的目标是： ？在动物肿瘤模型的各个方面提供咨询，包括肿瘤模型的创建和分析 并提供斯坦福大学研究人员正在使用的肿瘤模型数据库 ？通过胚胎和胚胎干细胞操作生产转基因和基因敲除小鼠 微量注射 ？为组织加工、染色和病理解释提供全面的组织病理学服务 ？肿瘤诱导、药物给药、毒性研究、数据的临床前肿瘤学服务 收集和分析 通过以下方式提供这些服务可节省大量成本、提高效率和实现科学进步 一个集中的设施。共享资源已经产生了500多个转基因和基因敲除小鼠模型 在过去的5年里，每一条都有多条独立的线路。超过24,000张组织学切片或组织块 2008年，共进行了670多例病理检查。共享资源的服务 在同行评议的期刊上发表的数百篇论文中得到认可或共同撰写。在日历中 2008年，代表十分之九的项目的癌症中心成员使用了共享资源。 共享资源的未来计划包括实施四倍体互补以加快 生产基因敲除小鼠，扩大组织病理学服务能力，并提供 复杂的肿瘤模型，如原位脑瘤、前列腺癌、胰腺癌或肾癌模型。

The Animal Tumor Models Shared Resource provides services in three major areas including transgenic and knockout mice, preclinical oncology and animal histopathology services. The transgenic and knockout mouse production services were established in 1996 at the School of Medicine with the overall objective of providing genetically modified mouse models and supporting technologies to Stanford investigators at cost-effective rates. The Veterinary Service Center (VSC) in the Department of Comparative Medicine runs the animal histopathology service component of the Shared Resource. Major services include tumor analysis by histology, immunohistochemistry, and pathological interpretation. The preclinical oncology services are new additions to the Animal Tumor Models Shared Resource. These services were funded and established by the Cancer Center to support the development and testing of new compounds and to better meet the growing needs of the Cancer Center members for preclinical animal models and therapeutics. The objectives of the Shared Resource are: ¿ Consultation in all aspects of animal tumor models, including creation and analysis of tumor models and providing a database of tumor models in use by Stanford investigators ¿ Production of transgenic and knockout mice by embryo and embryonic stem cell manipulation and microinjection ¿ Full histopathology services for tissue processing, staining and pathological interpretation ¿ Preclinical oncology services for tumor induction, drug administration, toxicity studies, data collection and analysis Substantial cost savings, efficiencies and scientific advances accrue from providing these services through a centralized facility. The Shared Resource has generated over 500 transgenic and knockout mouse models in the last 5 years, each with multiple independent lines. Over 24,000 histology slides or tissue blocks were processed and over 670 pathology procedures were performed in 2008. The Shared Resource's services were acknowledged or co-authored in hundreds of publications in peer-reviewed journals. In calendar year 2008, Cancer Center members representing nine out of ten Programs utilized the Shared Resource. Future plans for the Shared Resource include implementation of tetraploid complementation to speed up knockout mouse production, expansion of the capacity of histopathology services, and offer services on complex tumor models such as orthotopic brain, prostate, pancreatic or renal tumor models.