A NOVEL APPROACH TO GERM-LINE PRESERVATION IN MACAQUES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。目的：精原干细胞移植为扩增独特的基因型别提供了一种新的方法，也为产生转基因动物提供了一种新的方法。近期的目标是开发一种成功的方法来分离和丰富非人类灵长类生殖系前体细胞，并将来自遗传独特的雄性猕猴的精原干细胞移植到免疫缺陷小鼠和受体雄性猕猴。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: Spermatogonial stem cell transplantation may provide a method by which unique genotypes may be proliferated as well as a novel method to generate transgenic animals. Immediate objectives are to develop a successful method to isolate and enrich nonhuman primate germ line precursor cells and transplant spermatogonial stem cells from genetically unique male macaques to immunodeficient mice and recipient male macaques.