A NOVEL APPROACH TO GERM-LINE PRESERVATION IN MACAQUES

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。目的：精原干细胞移植可能提供一种独特基因型增殖的方法，也可能提供一种产生转基因动物的新方法。近期目标是开发一种成功的方法，分离和富集非人类灵长类动物生殖系前体细胞，并将来自遗传独特的雄性猕猴的精原干细胞移植到免疫缺陷小鼠和受体雄性猕猴体内。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: Spermatogonial stem cell transplantation may provide a method by which unique genotypes may be proliferated as well as a novel method to generate transgenic animals. Immediate objectives are to develop a successful method to isolate and enrich nonhuman primate germ line precursor cells and transplant spermatogonial stem cells from genetically unique male macaques to immunodeficient mice and recipient male macaques.