FUNDAMENTAL CRYOBIOLOGY OF MACAQUE SPERMATOZOA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 有效地保存恒河猴（Macaca mulatta）的遗传资源是国家资源研究中心的使命的关键。本申请旨在开发有效的猕猴精子冷冻保存，并将有助于通过人工授精和体外受精程序成功繁殖种质。非人灵长类动物（NHP），特别是遗传独特的猕猴，是人类疾病的重要模型，可应用于不孕不育和避孕，传染病和疫苗开发，药物和酒精成瘾，神经系统疾病和再生医学。与人类精子相似，来自NHP的冷冻保存的精子显示出不同的冷冻保护成功率，这取决于单个精子供体。精液供体通常是根据成功的冷冻保存结果选择的，然而，许多个体雄性，包括研究项目中的恒河猴雄性，其精液质量无法在冷冻保存中存活。这对于从胚胎干细胞、体细胞核移植和转基因产生的NHP可能尤其如此，其中需要特定个体基因型的繁殖。这项资助分析了一些可能导致低温保存效果不佳的机制，并重点研究了统一低温保存这些双峰种群的方法。我们的中心假设是，精子冷冻保存相关的生理反应的理解将导致精子冷冻存活的改善。我们的长期目标是了解精子冷冻保存的关键机制，以便最佳地保存来自各种基因型独特和有价值的NHP男性的精子。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Efficient preservation of genetic resources of the rhesus monkey (Macaca mulatta) is critical to the mission of the National Center for Research Resources. This application is aimed at the development of efficient macaque sperm cryopreservation and will aid in successful propagation of germplasm through artificial insemination and in vitro fertilization programs. Non-human primates (NHPs), especially genetically unique macaques, are important models of human disease with applications to infertility and contraception, infectious diseases and vaccine development, drug and alcohol addiction, neurological disorders, and regenerative medicine. Similar to human sperm, cryopreserved sperm from NHP's show variable cryoprotection success depending on the individual sperm donor. Semen donors are often selected based on successful cryopreservation results, however, many individual males, including rhesus males in research programs, have semen quality that fails to survive cryopreservation. This is likely to be particularly true concerning NHPs generated from embryonic stem cells, somatic cell nuclear transfer, and transgenics where propagation of a specific individual's genotype is desired. This grant analyzes some of the mechanisms that may cause poor cryoperformance and focuses on methods to uniformly cryopreserve these bimodal populations. Our central hypothesis is that an understanding of cryopreservation-associated physiological responses in sperm will lead to improvements in sperm cryosurvival. Our long-term goal is to develop an understanding of crucial mechanisms involved in cryopreservation of sperm in order to optimally preserve sperm from a variety of genotypically unique and valuable NHP males.