FUNDAMENTAL CRYOBIOLOGY OF MACAQUE SPERMATOZOA

猕猴精子的基础低温生物学

• 批准号: 7715573
• 负责人: STUART A MEYERS
• 金额: $ 5.42万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7715573
• 关键词: Alcohol dependence; Artificial Insemination; Biological Preservation; Communicable Diseases; Computer Retrieval of Information on Scientific Projects Database; Contraceptive methods; Cryopreservation; Development; Fertilization in Vitro; Funding; Genotype; Goals; Grant; Human; Individual; Infertility; Institution; Lead; Macaca; Macaca mulatta; Methods; Mission; Modeling; National Center for Research Resources; Pharmaceutical Preparations; Physiological; Population; Regenerative Medicine; Research; Research Personnel; Resources; Semen Donor; Seminal fluid; Source; Transgenic Organisms; United States National Institutes of Health; base; cryobiology; desire; embryonic stem cell; genetic resource; human disease; male; nervous system disorder; nonhuman primate; programs; response; somatic cell nuclear transfer; sperm cell; success; vaccine development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: Efficient preservation of genetic resources of the rhesus monkey (Macaca mullatta) is critical to the mission of the National Center for Research Resources. This application is aimed at the development of efficient macaque sperm cryopreservation and will aid in successful propagation of germplasm through artificial insemination and in vitro fertilization programs. Non-human primates (NHPs), especially genetically unique macaques, are important models of human disease with applications to infertility and contraception, infectious diseases and vaccine development, drug and alcohol addiction, neurological disorders, and regenerative medicine. Similar to human sperm, cryopreserved sperm from NHP's show variable cryoprotection success depending on the individual sperm donor. Semen donors are often selected based on successful cryopreservation results, however, many individual males, including rhesus males in research programs, have semen quality that fails to survive cryopreservation. This is likely to be particularly true concerning NHPs generated from embryonic stem cells, somatic cell nuclear transfer, and transgenics where propagation of a specific individual's genotype is desired. This grant analyzes some of the mechanisms that may cause poor cryoperformance and focuses on methods to uniformly cryopreserve these bimodal populations. Our central hypothesis is that an understanding of cryopreservation-associated physiological responses in sperm will lead to improvements in sperm cryosurvival. Our long-term goal is to develop an understanding of crucial mechanisms involved in cryopreservation of sperm in order to optimally preserve sperm from a variety of genotypically unique and valuable NHP males.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 目的：有效保存恒河猴（Macaca mullatta）遗传资源对于国家研究资源中心的使命至关重要。该应用旨在开发高效的猕猴精子冷冻保存，并将有助于通过人工授精和体外受精计划成功繁殖种质。非人类灵长类动物（NHP），特别是基因独特的猕猴，是人类疾病的重要模型，可应用于不孕症和避孕、传染病和疫苗开发、药物和酒精成瘾、神经系统疾病和再生医学。与人类精子类似，来自 NHP 的冷冻保存精子显示出不同的冷冻保护成功率，具体取决于个体精子捐献者。精液捐献者通常是根据成功的冷冻保存结果来选择的，然而，许多雄性个体，包括研究项目中的雄性恒河猴，其精液质量无法在冷冻保存中存活。对于胚胎干细胞产生的 NHP、体细胞核移植和需要繁殖特定个体基因型的转基因来说，这一点可能尤其正确。该资助分析了一些可能导致冷冻性能不佳的机制，并重点研究统一冷冻保存这些双峰群体的方法。我们的中心假设是，了解精子冷冻相关的生理反应将改善精子冷冻存活率。我们的长期目标是了解精子冷冻保存的关键机制，以便最佳地保存各种基因型独特且有价值的 NHP 男性的精子。