FUNDAMENTAL CRYOBIOLOGY OF MACAQUE SPERMATOZOA

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 目的：高效保存恒河猴(Macaca Mullatta)的遗传资源对于国家资源研究中心的使命至关重要。这一应用旨在开发高效的猕猴精子冷冻保存，并将有助于通过人工授精和体外受精计划成功繁殖种质。非人灵长类动物，特别是基因独特的猕猴，是人类疾病的重要模型，应用于不孕不育和避孕、传染病和疫苗开发、药物和酒精成瘾、神经疾病和再生医学。与人类精子相似，来自NHP的冷冻保存的精子显示出不同的冷冻保护成功，这取决于不同的精子捐赠者。捐精者通常是根据冷冻成功的结果来选择的，然而，许多个体男性，包括研究项目中的恒河猴，精液质量无法在冷冻后存活下来。对于胚胎干细胞产生的NHP，体细胞核移植，以及希望繁殖特定个体的基因的转基因，这一点可能尤其正确。这项资助分析了一些可能导致低温性能差的机制，并重点研究了统一低温保存这些双峰种群的方法。我们的中心假设是，了解精子中与冷冻保存相关的生理反应将有助于提高精子的冷冻存活率。我们的长期目标是了解精子冷冻保存的关键机制，以便以最佳方式保存来自各种基因独特和有价值的NHP男性的精子。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: Efficient preservation of genetic resources of the rhesus monkey (Macaca mullatta) is critical to the mission of the National Center for Research Resources. This application is aimed at the development of efficient macaque sperm cryopreservation and will aid in successful propagation of germplasm through artificial insemination and in vitro fertilization programs. Non-human primates (NHPs), especially genetically unique macaques, are important models of human disease with applications to infertility and contraception, infectious diseases and vaccine development, drug and alcohol addiction, neurological disorders, and regenerative medicine. Similar to human sperm, cryopreserved sperm from NHP's show variable cryoprotection success depending on the individual sperm donor. Semen donors are often selected based on successful cryopreservation results, however, many individual males, including rhesus males in research programs, have semen quality that fails to survive cryopreservation. This is likely to be particularly true concerning NHPs generated from embryonic stem cells, somatic cell nuclear transfer, and transgenics where propagation of a specific individual's genotype is desired. This grant analyzes some of the mechanisms that may cause poor cryoperformance and focuses on methods to uniformly cryopreserve these bimodal populations. Our central hypothesis is that an understanding of cryopreservation-associated physiological responses in sperm will lead to improvements in sperm cryosurvival. Our long-term goal is to develop an understanding of crucial mechanisms involved in cryopreservation of sperm in order to optimally preserve sperm from a variety of genotypically unique and valuable NHP males.