TESTING HBV-KOREA SINGLE VIAL FORMULATION FOR IMMUNOGENICITY RESPONSE IN BABOONS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a three-part study. The main objective of part one is to collect two lymph nodes and one skin biopsy from 6 baboons, and culture lymph node cells in vitro (for 6 hours) to test for background levels of gene products, using PCR analysis. The purpose of part two of this study is to determine if there is a correlate between the immune activity measured in vitro (Part 1) with in vivo activity in baboons following subcutaneous injection with 1018 ISS, 1018 ISS-PMXB, C274 and C792 formulations. For this part of the study 10 baboons will be added. The purpose of part three is to test the in vitro and in vivo responses of baboons to oligonucleotides. This experiment will determine if in vitro assays are possible for the detection of gene products of cellular activation after culture of cells with oligonucleotides/PMXB. The testing of these products has the potential to aid in the development of a treatment for asthma in humans. This study is currently in progress.