MEMBRANE TOPOLOGY OF MAMMALIAN P450

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。肝微粒体药物代谢系统负责氧化数百种外源性和内源性化合物。该体系由P450和P450还原酶组成。因此，决定P450活性的细胞和分子机制引起了极大的兴趣。该项目的目的是了解生产功能性P450分子的结构基础，包括适当靶向内质网，摄取血红素以及实现与其底物和还原酶相互作用兼容的膜拓扑结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Liver microsomal drug metabolizing system is responsible for oxidation of hundrends of exogeneous and endogeneous compounds. The system consists of P450 and P450 reductase. Therefore, the cellular and molecular mechanisms that determine the activity of P450 are great interest. The aim of project is to understand the structural basis for the production of a functional P450 molecule which includes proper targeting to endoplasmic reticulum, uptake of heme and achieving a membrane topology compatible with interaction with its substrates and reductase.