MEMBRANE TOPOLOGY OF MAMMALIAN P450

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。肝微体药物代谢系统负责外源性和内源性化合物的氧化。该系统由P450和P450还原酶组成。因此，决定P450活性的细胞和分子机制引起了人们极大的兴趣。该项目的目的是了解产生功能性P450分子的结构基础，包括适当的内质网靶向，对血红素的吸收，以及获得与其底物和还原酶相互作用的膜拓扑结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Liver microsomal drug metabolizing system is responsible for oxidation of hundrends of exogeneous and endogeneous compounds. The system consists of P450 and P450 reductase. Therefore, the cellular and molecular mechanisms that determine the activity of P450 are great interest. The aim of project is to understand the structural basis for the production of a functional P450 molecule which includes proper targeting to endoplasmic reticulum, uptake of heme and achieving a membrane topology compatible with interaction with its substrates and reductase.