PURINERGIC RECEPTOR RESEARCH

嘌呤能受体研究

• 批准号: 7358044
• 负责人: PAUL A INSEL
• 金额: $ 1.22万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358044
• 关键词: PURINERGIC; RECEPTOR; RESEARCH

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Epithelial cells along the collecting duct regulate free water reabsorption and acid secretion through various hormones and neurotransmitters, including atrial natriuretic peptide, bradykinin, catecholamines, miner-alocorticoids, prostanoids, and vasopressin. Under certain circumstances, P2-purinoceptor agonists (ATP, UTP, ADP, and UDP) can also regulate ion transport in these cells by activating various signaling cascades. Two types of P2-purinoceptors have been described to date: P2X-purinoceptors that correspond to ligand-gated cation channels and P2Y-purinoceptors that are coupled to heterotrimeric G proteins. Both the P2X- and P2Y-purinoceptors are known to occur in several isoforms, most of which exhibit wide tissue distribution. These proteins are also diffusely distributed along various nephron segments. Previous work suggests striking differences in expression of G protein coupled receptors on polarized MDCK cells but information regarding endogenously expressed purinergic P2Y receptor subtypes is not available. We are using the Bio-rad Radiance confocal microscope and Imaris software in order to examine the localization of various purinergic receptors in MDCK D-1 cell in order to detect green fluorescent protein (GFP) labeled P2Y purinergic receptors. Specifically, we are attempting to image sagital and transverse images of confluent cell layers.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。沿着集合管的上皮细胞沿着通过各种激素和神经递质调节游离水重吸收和酸分泌，所述激素和神经递质包括心房利钠肽、缓激肽、儿茶酚胺、盐皮质激素、前列腺素和加压素。在某些情况下，P2-嘌呤受体激动剂（ATP、UTP、ADP和UDP）也可以通过激活各种信号级联来调节这些细胞中的离子转运。迄今为止已经描述了两种类型的P2-嘌呤受体：对应于配体门控阳离子通道的P2 X-嘌呤受体和与异源三聚体G蛋白偶联的P2 Y-嘌呤受体。已知P2 X-和P2 Y-嘌呤受体以几种亚型存在，其中大多数表现出广泛的组织分布。这些蛋白质也沿沿着各种肾单位节段弥散分布。以前的工作表明，极化MDCK细胞的G蛋白偶联受体的表达显着差异，但内源性表达的嘌呤能P2 Y受体亚型的信息是不可用的。我们利用Bio-rad Radiance共聚焦显微镜和Imaris软件检测MDCK D-1细胞中各种嘌呤受体的定位，以检测绿色荧光蛋白（GFP）标记的P2 Y嘌呤受体。具体来说，我们正试图图像的矢状和横向图像的融合细胞层。