HETEROGENEITY OF DISTRIBUTION OF GAP JUNCTION PLAQUES IN THE MOUSE HEART

小鼠心脏间隙连接斑块分布的异质性

• 批准号: 7358116
• 负责人: Andrew D. McCulloch
• 金额: $ 0.2万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358116
• 关键词: HETEROGENEITY; DISTRIBUTION; GAP; JUNCTION; PLAQUES

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Electrical transfer between the myocytes within the heart occurs at the gap junctions. Two connexons in juxtaposed membranes link to form a gap junction. An assembly of six membrane spanning proteins called connexins from a connexon. Cardiac cells express various isoforms of connexins which have distinct biophysical properties. In order to study patterns of myocyte-myocyte connectivity, the aim of this project is to study the heterogeneity of distribution of gap junctional proteins such as connexin43 in the mouse heart. Measures of heterogeneity of distribution include computing regional variations within the heart and computing anisotropy of distribution about the cell. Since the gap junction plaques are only a few microns in dimension, we would like to use a confocal microscope to obtain the high-resolution images. Since a mouse heart is approximately 7mm in diameter and 1 cm long the tiled images will need to be montaged to obtain the full image.

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。心脏内肌细胞之间的电传递发生在间隙连接处。并置膜中的两个连接子连接形成间隙连接。连接子由六个膜跨越蛋白组成，称为连接蛋白。心肌细胞表达具有不同生物物理特性的各种连接蛋白同种异构体。为了研究心肌细胞-心肌细胞连接模式，本项目的目的是研究小鼠心脏间隙连接蛋白（如connexin43）分布的异质性。分布异质性的测量包括计算心脏内的区域变化和计算细胞分布的各向异性。由于间隙连接斑块的尺寸只有几微米，因此我们希望使用共聚焦显微镜获得高分辨率图像。由于老鼠心脏直径约7毫米，长1厘米，平铺图像需要蒙太奇才能获得完整的图像。