SINGLE-MOLECULE STUDY OF THE HETEROGENEITY OF SERPIN POLYMERIZATION

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. alpha-1-antitrypsin (AT) is the most abundantly circulating human proteinase inhibitor in the serpin family. The polymerization of AT, leading to alpha-1-antitrypsin deficiency, has been studied extensively in vitro by a variety of ensemble methods. We propose to study the polymerization reaction using newly developed single molecule techniques in our laboratory in combination with fluorescence spectroscopy.