SINGLE-MOLECULE STUDY OF THE HETEROGENEITY OF SERPIN POLYMERIZATION

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。α -1-抗胰蛋白酶（AT）是蛇形蛋白家族中循环最丰富的人类蛋白酶抑制剂。AT的聚合导致α -1-抗胰蛋白酶缺乏，已经通过各种集合方法在体外进行了广泛的研究。我们建议利用实验室新开发的单分子技术，结合荧光光谱技术来研究聚合反应。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. alpha-1-antitrypsin (AT) is the most abundantly circulating human proteinase inhibitor in the serpin family. The polymerization of AT, leading to alpha-1-antitrypsin deficiency, has been studied extensively in vitro by a variety of ensemble methods. We propose to study the polymerization reaction using newly developed single molecule techniques in our laboratory in combination with fluorescence spectroscopy.