Facilitated assembly of RNA pol III RNPs - biogenesis of the human SRP complex

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The function of all cells requires that individual activities are harnessed and controlled, often within large molecular machines. Examples include the machineries that replicate DNA, synthesize proteins and generate energy (ATP). These complexes must be assembled correctly in a controlled way, far from where they function, as partially or incorrectly assembled complexes might interfere with the normal processes. We wish to study the assembly of one such cellular machine, the signal recognition particle that is intimately associated with protein secretion from cells. Previous understanding indicates that this takes place within the nucleus, and largely within a subnuclear region termed the nucleolus, which is also the site of assembly of a number of other complexes, notably the protein synthesizing ribosome. Our aim is to identify components of the assembly and localisation path of signal recognition particle and to determine the role that each of these plays in these events.