CHROMATIN-ASSOCIATED PROTEIN COMPLEXES IN THE C ELEGANS GERM LINE

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。him -17是一种丰富的染色质相关蛋白，是秀丽隐杆线虫减数分裂前性染色体组蛋白修饰的正常补体和减数分裂重组起始所必需的。HIM-17是一种模块化蛋白，包含6个大而高度简并但保守的基序，可能作为DNA结合模块。为了了解HIM-17如何作为染色质修饰和减数分裂双链断裂形成之间的分子联系，我们希望使用免疫沉淀蛋白复合物的串联质谱法鉴定体内与HIM-17相关的蛋白质。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. HIM-17is an abundant chromatin-associated protein that is required both for initiation of meiotic recombination and for the normal complement of histone modifications on sex chromosomes during meiotic prophase in C. elegans. HIM-17 is a modular protein containing of six copies of a large and highly degenerate but conserved motif that likely functions as a DNA binding module. To understand how HIM-17 serves as a molecular link between chromatin modification and meiotic double-strand break formation, we wish to identify the proteins that associate with HIM-17 in vivo using tandem mass spectrometry of immunoprecipitated protein complexes.