CHROMATIN-ASSOCIATED PROTEIN COMPLEXES IN THE C ELEGANS GERM LINE

线虫种系中的染色质相关蛋白复合物

• 批准号: 7420800
• 负责人: ANNE M VILLENEUVE
• 金额: $ 0.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420800
• 关键词: chromatin; nucleoproteins; proteins

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. HIM-17is an abundant chromatin-associated protein that is required both for initiation of meiotic recombination and for the normal complement of histone modifications on sex chromosomes during meiotic prophase in C. elegans. HIM-17 is a modular protein containing of six copies of a large and highly degenerate but conserved motif that likely functions as a DNA binding module. To understand how HIM-17 serves as a molecular link between chromatin modification and meiotic double-strand break formation, we wish to identify the proteins that associate with HIM-17 in vivo using tandem mass spectrometry of immunoprecipitated protein complexes.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。HIM-17是一种丰富的染色质相关蛋白，它是C减数分裂重组启动和减数分裂前期性染色体上组蛋白修饰正常补充所需的。优雅的HIM-17是一种模块化蛋白，含有六个拷贝的大的高度简并但保守的基序，其可能作为DNA结合模块发挥作用。为了了解HIM-17如何作为染色质修饰和减数分裂双链断裂形成之间的分子联系，我们希望使用免疫沉淀蛋白复合物的串联质谱法鉴定与HIM-17在体内相关的蛋白质。