Cryopreservation of stem cells

干细胞的冷冻保存

• 批准号: BB/D014751/1
• 负责人: Zhanfeng Cui
• 金额: $ 57.78万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2006
• 资助国家: 英国
• 起止时间: 2006 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FD014751%2F1
• 关键词: Cryopreservation; stem; cells

Stem cells are special type of cells which can renew themselves, and can grow into many other cell types. Scientists have found stem cells not only in embryo (so called embryonic stem cells) but also in adults (adult stem cells, e.g from bone marrow). Stem cells could be very useful for tissue/organ repair (e.g brain, heart and other tissue). But in order to apply stem cell based therapies into clinic settings, many technical obstacles must be overcome. One of these is how to preserve stem cells for long term. The most feasible method is cryopreservation. For example, when a baby is born, the stem cells in the cord blood can be collected, separated, frozen and stored at liquid nitrogen temperature (-196 oC). If in later stage in his (or her) life the individual needs stem cells, his (or her) cells can be thawed and (appropriate) therapy can be started. Other types of stem cells can similarly be preserved. The problem is that stem cells do not like low temperatures (not a surprise). They may simply die, or lose some important functions. The additional complexity is that it is not easy to tell what/how many functions are lost. This project focuses on two important questions (1) how to make sure stem cell functions be preserved by using better cryopreservation agents (e.g use of antfreeze proteins protecting fishes in arctic) and thereby adjusting how fast we freeze the cells, and (2) how to detect any damage by using new type of microscope to three dimensionally see inside and around the cells. At the end of the project, we hope we will have optimal cryoprotecting chemical formulation and optical protocols, which can be used by stem cell banks, research laboratories, hospitals and industry.

干细胞是一种特殊类型的细胞，可以自我更新，并可以成长为许多其他类型的细胞。科学家们不仅在胚胎中发现了干细胞(所谓的胚胎干细胞)，而且在成人中也发现了干细胞(成人干细胞，例如来自骨髓的干细胞)。干细胞对组织/器官修复(如大脑、心脏和其他组织)非常有用。但为了将基于干细胞的疗法应用到临床环境中，必须克服许多技术障碍。其中之一就是如何长期保存干细胞。最可行的方法是冷冻保存。例如，当婴儿出生时，脐带血中的干细胞可以被收集、分离、冷冻并在液氮温度(-196摄氏度)下储存。如果在他(或她)生命的后期需要干细胞，他(或她)的细胞可以被解冻，(适当的)治疗可以开始。其他类型的干细胞同样可以保存下来。问题是干细胞不喜欢低温(这并不令人惊讶)。它们可能会干脆死亡，或者失去一些重要的功能。额外的复杂性是，很难判断丢失了哪些功能/多少功能。这个项目集中在两个重要问题上(1)如何通过使用更好的冷冻剂(例如使用抗冻蛋白保护北极的鱼类)来确保干细胞的功能得到保存，从而调整我们冷冻细胞的速度，以及(2)如何通过使用新型显微镜来三维观察细胞内部和周围来检测任何损害。在项目结束时，我们希望我们将有最优的低温保护化学配方和光学方案，可以用于干细胞库、研究实验室、医院和工业。

项目成果

Effect of various freezing solutions on cryopreservation of mesenchymal stem cells from different animal species.

不同冷冻溶液对不同动物种间充质干细胞冻存的影响。

• 发表时间: 2011
• 期刊: Cryo letters
• 作者: Liu Y

RGDS-fuctionalized alginates improve the survival rate of encapsulated embryonic stem cells during cryopreservation.

RGDS 功能化海藻酸盐可提高冷冻保存过程中封装胚胎干细胞的存活率。

• 发表时间: 2011
• 期刊: Cryo letters
• 作者: Sambu S

Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation.

冷冻保存后解离的人类胚胎干细胞的细胞恢复增强。

• DOI: 10.1002/btpr.358
• 发表时间: 2010-05
• 期刊: BIOTECHNOLOGY PROGRESS
• 影响因子: 2.9
• 作者: Xu, Xia;Cowley, Sally;Flaim, Christopher J.;James, William;Seymour, Lenard W.;Cui, Zhanfeng
• 通讯作者: Cui, Zhanfeng

The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells.

• DOI: 10.1002/btpr.368
• 发表时间: 2010-05
• 期刊: BIOTECHNOLOGY PROGRESS
• 影响因子: 2.9
• 作者: Xu, Xia;Cowley, Sally;Flaim, Christopher J.;James, William;Seymour, Leonard;Cui, Zhanfeng
• 通讯作者: Cui, Zhanfeng