权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Anterograde and retrograde transport of the plant vacuolar sorting receptor BP80 in vivo

植物液泡分选受体BP80体内的顺行和逆行转运

基本信息

批准号：
BB/D016223/1
负责人：
Jurgen Denecke
金额：
$ 38.92万
依托单位：
University of Leeds
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2006
资助国家：
英国
起止时间：
2006 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FD016223%2F1
关键词：
Anterograde retrograde transport plant vacuolar

项目摘要

Understanding how proteins are sorted to the right place in a living cell is comparable to the task of the Royal Mail to first sort letters and packages by destination and secondly to deliver them correctly at minimal cost and in a reasonable timeframe. This also means that vehicles should not travel when they are not properly loaded, and they should be doing something useful when they return, such as bringing some mail back. This is not an easy task, and the same is true for the sorting of proteins in living cells. Numerous protein sorting signals (address labels) have been described in the last 20 years and in many cases receptor molecules (drivers with instructions) have been identified that bind to the sorting signals. However, much less is known about the sorting of the sorting receptors themselves, in other words how do the drivers reach their right destination, and what do they do when they have arrived? In the cell, receptors must not only bind to ligands in one compartment, they must also transport them to another compartment, release them there, and return back to the original compartment to select new proteins. This process is extremely complex, because it means that receptor should only start transporting when they have bound to the right protein and when the transport carrier has been filled with appropriate cargo. On the other hand, receptors must release the proteins at the right place, and then return with a transport carrier for a new job. This project aims to understand how the plant vacuolar sorting receptor BP80 accomplishes the difficult task of finding its way in the cell and transport proteins to the right place in an efficient manner. We have already started to understand how receptors move forward, but we wish to find out more about these routes and also discover how and when they return from their trips.

了解蛋白质如何在活细胞中被分类到正确的位置就像皇家邮政的任务一样，首先按目的地对信件和包裹进行分类，然后以最低的成本在合理的时间内正确地将它们投递。这也意味着车辆在未正确装载的情况下不应行驶，并且在返回时应该做一些有用的事情，例如带回一些邮件。这不是一件容易的事，对于活细胞中蛋白质的分选也是如此。在过去的 20 年里，人们描述了许多蛋白质分选信号（地址标签），并且在许多情况下，已经鉴定出与分选信号结合的受体分子（带有指令的驱动程序）。然而，人们对分拣接收器本身的分拣知之甚少，换句话说，司机如何到达正确的目的地，以及到达后他们会做什么？在细胞中，受体不仅必须与一个区室中的配体结合，还必须将配体运输到另一个区室，在那里释放它们，然后返回到原来的区室以选择新的蛋白质。这个过程非常复杂，因为这意味着受体只有在与正确的蛋白质结合并且运输载体已装满适当的货物时才应开始运输。另一方面，受体必须在正确的位置释放蛋白质，然后带着运输载体返回以进行新的工作。该项目旨在了解植物液泡分选受体 BP80 如何完成在细胞中找到路径并以有效方式将蛋白质转运到正确位置的艰巨任务。我们已经开始了解受体如何前进，但我们希望了解更多有关这些路线的信息，并了解它们如何以及何时从旅行中返回。