STRUCTURAL STUDIES OF METALLOPROTEINS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。金属在许多蛋白质的功能中起着不可或缺的作用。这些金属蛋白的结构测定是阐明金属介导其生物过程的分子机制的基础。这项建议主要集中在三个主要领域的金属酶的结构研究：(1)由乙酸盐和甲胺生成甲基的产甲烷蛋白的结构测定，(2)新型金属蛋白酶的结构测定，以及(3)蛋白质氧传感器的结构测定。每个逆冲区的一些蛋白质已经结晶，项目目前正处于其结构解决方案的不同阶段。未来研究的重点将是利用在SSRL光束线上收集的MAD和MIR数据完成正在进行的结构测定，并确定这些蛋白质的抑制剂和中间复合体的结构，以澄清与机制和配体结合相关的问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Metals play an integral role in the function of many proteins. The structural determination of these metalloproteins is fundamental for elucidating the molecular mechanisms by which metals mediate their biological processes. This proposal focuses on structural studies of metalloenzymes in three major areas: (1) the structural determination of methanogenic proteins involved in generating the methyl group from acetate and methylamines, (2) the structural determination of novel metalloproteases, and (3) the structural determination of protein oxygen sensors. A number of proteins in each thrust area have been crystallized and projects are currently at various stages towards their structure solution. The focus of future studies will be to complete ongoing structural determinations using MAD and MIR data collected on SSRL beamlines and to determine the structures of inhibitor and intermediate complexes of these proteins to clarify issues related to mechanism and ligand binding.