CRYSTAL STRUCTURES OF CLIC ION CHANNELS, SERPIN COMPLEXES, RNA BINDING PROTEINS

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our proposal looks at the structures of several protein systems, in each case, we aim to map the functionally important structural transitions that occur in the protein. CLIC1 is a member of a new class of chloride ion channel. These proteins exist in both a soluble and integral membrane forms. We have just solved the first structure of CLIC1 in the soluble form and we have crystals of the first intermediate state in the transition to the membrane bound form. The Sm/Lsm proteins form the basis for many RNPs in eucaryotic cells (including the spliceosome, telomerase and degradosome). They are also present in archaea. We have completed the structure of an archaeal Lsm complex & have recently formed a complex with RNA. We wish to determine this structure and higher order complexes. PE545 is a 50 kDa light harvesting protein that crystallizes in a form that diffracts to at least 0.86 ¿¿ We have solved this structure at 1.1 ¿¿and aim to improve this to maximal resolution. We also have crystals of prefoldins, chaperonins and serpins.