CRYSTAL STRUCTURES OF CLIC ION CHANNELS, SERPIN COMPLEXES, RNA BINDING PROTEINS

CLIC 离子通道、丝氨酸蛋白酶抑制剂复合物、RNA 结合蛋白的晶体结构

• 批准号: 7370362
• 负责人: PAUL CURMI
• 金额: $ 0.3万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370362
• 关键词: CRYSTAL; STRUCTURES; CLIC; ION; CHANNELS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our proposal looks at the structures of several protein systems, in each case, we aim to map the functionally important structural transitions that occur in the protein. CLIC1 is a member of a new class of chloride ion channel. These proteins exist in both a soluble and integral membrane forms. We have just solved the first structure of CLIC1 in the soluble form and we have crystals of the first intermediate state in the transition to the membrane bound form. The Sm/Lsm proteins form the basis for many RNPs in eucaryotic cells (including the spliceosome, telomerase and degradosome). They are also present in archaea. We have completed the structure of an archaeal Lsm complex & have recently formed a complex with RNA. We wish to determine this structure and higher order complexes. PE545 is a 50 kDa light harvesting protein that crystallizes in a form that diffracts to at least 0.86 ¿¿ We have solved this structure at 1.1 ¿¿and aim to improve this to maximal resolution. We also have crystals of prefoldins, chaperonins and serpins.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。我们的建议着眼于几种蛋白质系统的结构，在每种情况下，我们的目标是绘制蛋白质中发生的功能上重要的结构转变。 CLIC1 是一类新型氯离子通道的成员。这些蛋白质以可溶性和完整膜形式存在。我们刚刚解决了可溶形式的 CLIC1 的第一个结构，并且我们得到了向膜结合形式过渡的第一个中间态的晶体。 Sm/Lsm 蛋白构成真核细胞中许多 RNP（包括剪接体、端粒酶和降解体）的基础。它们也存在于古细菌中。我们已经完成了古菌 Lsm 复合体的结构，并且最近与 RNA 形成了复合体。我们希望确定这种结构和高阶配合物。 PE545 是一种 50 kDa 的光捕获蛋白，其结晶形式的衍射度至少为 0.86 — 我们已在 1.1 级解析了该结构，并旨在将其提高到最大分辨率。我们还有前折叠蛋白、伴侣蛋白和丝氨酸蛋白酶抑制剂的晶体。