Information-Rich Photon Imaging of Cells

点击翻译按钮获取中文摘要

Cells and tissue are complex materials whose heterogeneity and dynamic changes are principally driven at the micron and sub-micron size scales, with underlying events ranging upwards from microsecond time scales, and measurement also based upon faster photo-electronic events of molecules (eg fluorescent and Raman emission from picosecond to nanosecond timescales). Advances in high energy physics and space research have made possible a fast photon imaging detector with >1000 channels each measuring individual photon arrivals to picosecond temporal sensitivity. We aim to develop and use this imaging detector to capture the high spatial and temporal complexity of cells and tissue. In particular, our objectives concern maximising the photon information available to unravel complex multi-component signals, including within background noise, below the visibility of present measurement and automated platforms, which underlie challenges in quantitative imaging and bioassays of live cells and tissue.