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Design of a novel photonic biosensor based on whispering-gallery modes of dielectric microspheres for high-throughput immunoassays

设计一种基于介电微球回音壁模式的新型光子生物传感器，用于高通量免疫分析

基本信息

批准号：
BB/E005624/1
负责人：
Paola Borri
金额：
$ 10.46万
依托单位：
CARDIFF UNIVERSITY
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2007
资助国家：
英国
起止时间：
2007 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FE005624%2F1
关键词：
Design novel photonic biosensor based

项目摘要

Our goal is to develop a new generation of label-free biosensor using a simple, versatile, and highly sensitive optical method. During the last decade, label-free optical biosensors have become valuable tools for clinical and military use as well as drug discovery. They are important devices for a range of applications going from detection of infectious agents, toxins, proteins and DNA to investigation of whole cell behaviour (e.g. attachment, spreading and proliferation of animal cells on solid surfaces), to quantitative determination of binding affinities and interactions kinetics between molecules. Modern label-free optical biosensors, like the widely utilised surface plasmon resonance (SPR) method, are based on an evanescent light field travelling along a planar surface, probing the target material deposited on the surface. A more sensitive type of biosensor based on evanescent field coupling can be realized by exploiting narrow-linewidth photonic resonances of dielectric microspheres, the so called whispering-gallery modes (WGMs), where the field is confined close to the inner surface of the sphere and is travelling around the sphere via total internal reflection. The light can orbit many thousand times before escaping the resonator, giving rise to an effective interaction length between the evanescent light field and the target molecules many orders of magnitude longer than the physical length on the sphere surface where molecules are deposited (its SPR analogous would correspond to a planar chip of more than one meter length!). This method should show unprecedented sensitivity but to fully explore the range of possible applications a proper theoretical model is still missing. We have recently commenced the construction and experimental study of a prototype WGM biosensor for immunosensing based on antibody-antigen interaction where many microspheres coated with different antibodies can be addressed in parallel via their evanescent coupling to planar waveguide modes propagating near a substrate surface. With the present project we want to develop a theoretical modelling to assess the applicability of this optical biosensor toward sensitive detection not only of proteins but also of viruses and bacteria. By combining this rigorous theoretical study with the experiments already ongoing we aim to realize the design of an optimum label-free biosensor for high-throughput immunoassays.

我们的目标是开发新一代的无标记的生物传感器，使用一个简单的，通用的，和高灵敏度的光学方法。在过去的十年中，无标记光学生物传感器已成为临床和军事用途以及药物发现的宝贵工具。它们是一系列应用的重要设备，从检测感染因子、毒素、蛋白质和DNA到研究全细胞行为（例如动物细胞在固体表面上的附着、扩散和增殖），再到定量测定分子之间的结合亲和力和相互作用动力学。现代的无标记光学生物传感器，如广泛使用的表面等离子体共振（SPR）方法，基于沿平坦表面沿着行进的渐逝光场，探测沉积在表面上的目标材料。一种更灵敏的基于倏逝场耦合的生物传感器类型可以通过利用电介质微球的窄线宽光子共振（所谓的回音壁模式（WGM））来实现，其中场被限制在接近球体的内表面并且通过全内反射围绕球体行进。光在逃离谐振器之前可以绕轨道运行数千次，从而引起倏逝光场与目标分子之间的有效相互作用长度比分子沉积在球表面上的物理长度长许多数量级（其SPR类似物将对应于超过一米长的平面芯片！）。这种方法应该表现出前所未有的灵敏度，但要充分探索可能的应用范围，仍然缺少适当的理论模型。我们最近已经开始建设和实验研究的原型WGM生物传感器的免疫传感的抗体-抗原相互作用的基础上，许多微球涂有不同的抗体可以通过其倏逝波耦合到平面波导模式传播附近的基板表面并行处理。通过本项目，我们希望开发一个理论模型来评估这种光学生物传感器不仅对蛋白质而且对病毒和细菌进行灵敏检测的适用性。通过结合这一严格的理论研究与实验已经在进行中，我们的目标是实现一个最佳的无标记生物传感器的高通量免疫分析的设计。