CONSENT TO DRAW BLOOD FOR THE EVALUATION OF GHRELIN

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。非天然的五肽GHRPs出现于1976年。1984年发现GHRP-GHRH在大鼠体内具有协同释放GH的作用，表明这两种多肽具有互补和独立的作用。GHRP被假设为反映一种新的下丘脑激素的活性。到1989年，GHRP-GH在人体内的释放显著增加，到1996年，GHRP-GHS受体被克隆。天然荷尔蒙Grehlin已经由Kojima等人分离出来。它的化学成分和主要的解剖来源是新的，因为它是一种由Ser3-辛酸降解的28个氨基酸的多肽，似乎起源于胃，但也存在于下丘脑尖锐核的腹侧缘。我们在大鼠身上的初步结果支持，天然的Ghrelin具有与非天然的GHRP相同的GH释放活性。在大鼠、狗和人身上的体外和体内研究已经被提出如下假说：1)Ghrelin是生理内源性的GHRP激素，2)Ghrp-2和Ghrelin基本上具有相同的生物学作用，3)天然的Ghrp、Ghrelin缺乏是一些正常老年人GH分泌减少的原因。我们现在有了测量Ghrelin基础水平的方法。我们已经使用了商业RIA试剂盒，并试图在我们的实验室建立RIA来检测血浆/血清中的Ghrelin水平。这将使我们能够确定不同个体在一天中不同时间、用餐前和用餐后的循环Ghrelin水平，以便评估受试者之间这种肽水平的精确度和差异。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The unnatural pentapeptide GHRPs appeared in 1976. GHRP-GHRH was found to synergistically release GH in rats in 1984, indicating the peptides' complementary and independent actions. GHRP was hypothesized to reflect the activity of a new hypothalmic hormone. By 1989, the GHRP GH release in humans was notable, and, by 1996, the GHRP-GHS receptor was cloned. The natural hormone, grehlin, has been isolated by Kojima et al. The chemistry and the major anatomical site of origin are novel since it is a Ser3-octanolyated 28 amino acid peptide that appears to originate in the stomach but also is present in the ventral lateral border of the hypothalamic acuate nucleus. Our preliminary results in rats support that natural ghrelin has the same GH releasing activity as the biological actions of unnatural GHRP. In vitro and in vivo studies in rats, dogs, and humans have been proposed to pursue the hypothesis that 1) ghrelin is the physiological endogenous GHRP hormone, 2) GHRP-2 and ghrelin essentially have the same biological actions, and 3) natural GHRP, ghrelin, deficiency is responsible for the decreased GH secretion of some normal older humans. We now have methods to measure the basal levels of ghrelin. We have used commercial RIA kits and are attempting to establishan RIA in our laboratory to determine ghrelin levels in plasma/serum. This will enable us to ascertain the levels of circulating ghrelin of different individuals at various times of the day, before and after meals, in order to assess the precision and differences in the levels of this peptide between subjects.