INTEGRATED MICROFLUIDIC SYSTEM FOR ORAL DIAGNOSTICS

用于口腔诊断的集成微流体系统

• 批准号: 7376619
• 负责人: WILLIAM V GIANNOBILE
• 金额: $ 4.19万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-05 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7376619
• 关键词: fluid; microfluidics; saliva; tooth

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study is being conducted to test the ability and accuracy of a new instrument in the diagnosis of periodontitis (gum disease) by using fluids from the mouth. The diagnosis instrument will test two types of fluids, gingival crevicular fluid (GCF) and saliva. GCF is the fluid that accumulates between your teeth and gum tissue. The fluids will be collected in three ways: 1. Direct GCF sampling: Thin paper films will be inserted into the space between the teeth and gums to absorb the fluid; 2. GCF rinse sampling: The patient will rinse with saline solution (water with salt content concentration similar to biological fluids) and spit into a tube; 3. Whole saliva: The patient will spit saliva into a tube. A total of 100 adult patients will participate in this study. These subjects will be of two patient populations: 1) diseased population, with active gum disease; and 2) a low-risk disease population, with no active gum disease. All subjects will have at least 20 teeth and not have received periodontal treatment or antibiotic-related therapy for medical or dental reasons 3 months prior to study inclusion. Patients will also be excluded from the study if they receive long-term use of medications known to affect periodontal status such as anti-inflammatory drugs, aspirin, and ibuprofen. The patients will also possess no history of metabolic bone diseases such as rheumatoid arthritis or post-menopausal osteoporosis. Patients will be seen 6 times over the course of the study over a period of approximately 12 months. At each visit the patient will undergo oral fluid collection, as previously described, and thorough examinations of the mouth, including the taking of measurements for study purposes. Patients will also have CT/radiographs taken at baseline, month 6, and end of study visits. At the end of the oral fluid collection period subjects will receive a deep cleaning (scaling and root planing) or a regular cleaning (prophy), dependent upon patient s needs.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。这项研究正在进行测试的能力和准确性的一种新的仪器在牙周炎（牙龈疾病）的诊断使用流体从口腔。诊断仪器将测试两种类型的液体，龈沟液（GCF）和唾液。GCF是积聚在牙齿和牙龈组织之间的液体。液体将通过三种方式收集：1.直接GCF采样：将薄纸膜插入牙齿和牙龈之间的空间以吸收液体; 2. GCF冲洗取样：患者将用盐水溶液（盐含量浓度类似于生物流体的水）冲洗并吐入管中; 3.全唾液：患者将唾液吐到试管中。共有100例成人患者将参与本研究。这些受试者将属于两个患者人群：1）患有活动性牙龈疾病的患病人群;和2）没有活动性牙龈疾病的低风险疾病人群。所有受试者将至少有20颗牙齿，并且在研究入选前3个月未因医学或牙科原因接受牙周治疗或牙周相关治疗。如果患者长期使用已知会影响牙周状况的药物，如抗炎药、阿司匹林和布洛芬，则也将被排除在研究之外。患者也没有代谢性骨疾病史，如类风湿性关节炎或绝经后骨质疏松症。在约12个月的研究期间，患者将接受6次访视。在每次访视时，患者将接受如前所述的口腔液体收集，并对口腔进行全面检查，包括出于研究目的进行测量。患者还将在基线、第6个月和研究结束访视时接受CT/X线片检查。在口腔液体收集期结束时，受试者将根据患者的需求接受深度清洁（洁治和根面平整）或定期清洁（预防）。