PHYSIOLOGICAL IMPORTANCE OF GROWTH HORMONE PULSATILITY

生长激素脉动的生理重要性

• 批准号: 7376507
• 负责人: CRAIG A JAFFE
• 金额: $ 20.54万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-05 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7376507
• 关键词: biopsy; birth; blood; blood glucose; bone; breath tests; diagnosis; erythromycin; glucose; glucose tolerance test; hypoglycemia; insulin; lead; lipids; liver; muscle; plasma; saline; somatotropin; urine

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In adults with growth hormone (GH) deficiency, GH replacement therapy is being increasingly utilized. GH is usually administered to patients as a daily subcutaneous injection. This leads to high GH levels that slowly decrease over several hours. However, in normal individuals GH is secreted in pulses and the GH levels fluctuate throughout the day. In this study we will address the question of whether the pattern of GH levels is important for GH action. We will study thirty adults with GH deficiency. The diagnosis will be confirmed by insulin hypoglycemia test. During this test a dose of insulin is given intravenously to decrease the blood glucose levels and cause symptoms of hypoglycemia. The GH response is then measured. The subjects will be admitted to the GCRC for 8 days three times. They will be studied during normal saline infusion, and during GH administration as a continuous intravenous infusion during one admission and as 6 separate intravenous doses per day during the other admission. The response of the liver will be tested by doing an erythromycin breath test, and measuring plasma lipid levels. The response of the bones will be assessed by several blood and urine tests, and the response of the muscles will be assessed by doing a needle biopsy of a thigh muscle. The effects of the different patterns of GH on glucose and insulin levels will be studied by intravenous glucose tolerance test. The results of this study will be important in the design of better GH delivery methods.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在生长激素（GH）缺乏的成年人中，GH替代疗法越来越多地被使用。GH通常作为每日皮下注射给予患者。这导致高GH水平，在几个小时内缓慢下降。然而，在正常个体中，GH以脉冲形式分泌，并且GH水平在一天中波动。在这项研究中，我们将解决的问题是否GH水平的模式是重要的GH行动。我们将研究30名患有GH缺乏症的成年人。将通过胰岛素低血糖试验确认诊断。在此测试期间，静脉注射一定剂量的胰岛素以降低血糖水平并引起低血糖症状。然后测量GH反应。受试者将分三次入住GCRC，为期8天。将在生理盐水输注期间和GH给药期间进行研究，在一次入院期间连续静脉输注GH，在另一次入院期间每天6次单独静脉给药。将通过进行红霉素呼吸试验和测量血浆脂质水平来检测肝脏的反应。骨骼的反应将通过几项血液和尿液检查进行评估，肌肉的反应将通过大腿肌肉的针吸活检进行评估。将通过静脉葡萄糖耐量试验研究不同GH模式对葡萄糖和胰岛素水平的影响。这项研究的结果将是重要的设计更好的生长激素输送方法。