SERUM PROTEOMIC PATTERNS IN NECROTIZING ENTEROCOLITIS IN PRETERM INFANTS

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。坏死性小肠结肠炎（NEC）是一种与早产儿肠壁缺血和坏死相关的疾病。它是影响早产儿的最常见的长期发病率之一。在< 1000 g的婴儿中，NEC的发生率为7%。该疾病的病因是多因素的;肠壁缺血、肠道细菌定植和进食的存在通常在病症发作之前存在。我们目前对NEC的分子发病机制的理解是有限的。 本研究的目的是利用蛋白质组学和生物信息学技术：1）鉴定使婴儿易患NEC的血清低分子量蛋白质生物标志物，2）鉴定参与NEC炎症级联反应的低分子量蛋白质。 在开始喂养前、NEC样症状发作时和NEC发作后24小时，从早产母亲和3天和7日龄早产婴儿中采集血液和脐带血。样品将在-70 ℃下储存，同时使用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）进行分析。将分析由样品中存在的数千种蛋白质中的一小部分蛋白质或肽形成的鉴别模式，以建立最可能与NEC相关的特征模式。本研究的结果将应用于设盲样本，作为大型多中心试验的一部分。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Necrotizing enterocolitis (NEC) is a condition that is associated with ischemia and necrosis of the bowel wall in preterm infants. It is one of the most common long term morbidities affecting premature infants. The incidence of NEC is 7% in < 1000 g infants. The etiology of the disease is multifactorial; bowel wall ischemia, bacterial colonization of the intestine and presence of feedings are usually present prior to the onset of the condition. Our current understanding of the molecular pathogenesis of NEC is limited. The goal of this study is use proteomics and bioinformatic technologies to: 1) identify serum low molecular weight protein biomarkers that predispose an infant to NEC, and 2) to identify low molecular weight proteins that participate in the inflammatory cascade in NEC. Blood will be collected from mothers in preterm labor and cord blood from preterm infants at 3 and 7 days of age, prior to initiation of feeds, at onset of NEC-like symptoms and 24 hrs after onset of NEC. Samples will be stored at -70C and analyzed simultaneously using surface enhanced, laser desorption ionization time of flight mass spectroscopy (SELDI-TOF-MS). The discriminating pattern formed by a small subset of proteins or peptides among the thousands of proteins present in the sample will be analyzed to establish a signature pattern that is most likely to be associated with NEC. Results from this study will be applied to masked samples as a part of a larger multicenter trial.