EFFECTS OF DIFFERENT BREAST PUMPING PROTOCOLS ON ALPHA LACTALBUMIN MRNA CONCE

不同吸奶方案对 α 乳清蛋白 mRNA 浓度的影响

• 批准号: 7375008
• 负责人: MOREY W HAYMOND
• 金额: $ 0.47万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7375008
• 关键词: EFFECTS; DIFFERENT; BREAST; PUMPING; PROTOCOLS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Over the last two decades, renewed interest and expertise in and about breastfeeding has occurred in the U.S. and Western Europe. Human milk is universally accepted as the ideal food for infants and breastfeeding has real and potential benefits for the mother, the infant and to their relationship. In the United States about 50% of women choose to breastfeed their infants despite all of the real and potential advantages to both the infant and mother. A number of these women have been unsuccessful in their attempts to breastfeed (insufficient milk syndrome) and others are known to be at high risk of breastfeeding failure (women with premature infants and teenage mothers). The primary determinant of milk volume is lactose production. We have previously demonstrated that following a 24 hr fast, lactating women have a 35% higher rate of glucose turnover than controls, tolerate a 24 hr fast without difficulty and have no differences in substrate or hormone concentrations when compared to controls. In addition, we demonstrated: 1. the human breast was capable of de novo synthesis of both the glucose and galactose moieties of lactose (hexoneogenesis), 2. The fraction of lactose derived from hexoneogenesis increased from 30% to nearly 50% comparing the fed to the 24 hr fasted values, 3. a source of amino acids other than the plasma free amino acid pool contributes to milk protein synthesis, and 4. human growth hormone (rhGH) increases human milk production and hepatic gluconeogenesis. Finally we demonstrated that milk fat globules contain sufficient specific mRNA to permit investigations of the substrate and hormonal factors that regulate gene expression in the human lactating breast. Understanding whether and how the mRNA in the milk fat globules are regulated is the initial focus of these studies. Because of our previous experience with rhGH, we will initially test the acute effect of rhGH on alpha lactalbumen mRNA expression in milk fat globules. Over all, research program will build on our current knowledge and techniques to provide indepth information on the maternal adaptation to lactation and the regulation of lactose production. By better understanding the factors regulating lactose production, we will be better able to design interventional strategies for individuals who struggle with successful breast feeding.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。在过去的二十年里，美国和西欧对母乳喂养重新产生了兴趣和专业知识。母乳被普遍认为是婴儿的理想食物，母乳喂养对母亲、婴儿和他们的关系都有真实的和潜在的好处。在美国，尽管母乳喂养对婴儿和母亲都有真实的和潜在的好处，但大约50%的妇女选择母乳喂养婴儿。其中一些妇女尝试母乳喂养没有成功（奶水不足综合症），其他人则有很高的母乳喂养失败风险（有早产儿的妇女和少女母亲）。乳量的主要决定因素是乳糖产量。我们之前已经证明，在禁食24小时后，哺乳期妇女的葡萄糖周转率比对照组高35%，耐受24小时禁食没有困难，与对照组相比，底物或激素浓度没有差异。此外，我们还论证了：1.人乳房能够从头合成乳糖的葡萄糖和半乳糖部分（己酮生成），2.与24小时禁食值相比，来自己酮生成的乳糖分数从30%增加到近50%，3。血浆游离氨基酸库以外的氨基酸源有助于乳蛋白合成，和4.人生长激素（rhGH）增加母乳产量和肝再生。最后，我们证明，乳脂球含有足够的特定mRNA，允许调查的基板和激素因子，调节基因表达的人类哺乳期乳房。了解是否以及如何在乳脂球的mRNA的调节是这些研究的最初重点。由于我们以前使用rhGH的经验，我们将首先测试rhGH对乳脂球中α乳白蛋白mRNA表达的急性影响。 总的来说，研究计划将建立在我们现有的知识和技术，提供深入的信息，产妇适应哺乳和乳糖生产的调节。通过更好地了解调节乳糖产生的因素，我们将能够更好地为那些难以成功母乳喂养的人设计干预策略。