RACIAL DIFFERENCES IN CIRCULATING SEX STEROIDS: MECHANISMS AND EFFECT ON BONE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。这项研究的目的是比较黑人女性和白人女性在整个月经周期中类固醇水平的特征，并监测肥胖和种族对性类固醇水平、骨密度和结构的影响。似乎有越来越多的有力证据表明，循环性类固醇水平存在根本性差异，这是导致黑人终末器官变化的原因之一。我们建议更详细地记录这些数据，最重要的是考虑到不同种族群体之间的肥胖差异。此外，到目前为止，除了描述性研究之外，什么都没有，对潜在机制的探索很少。在这项研究中，200名黑人和白人血统的正常骑自行车的女性将被研究。我们将深入研究月经周期，每天收集尿液，并对一个周期进行抽血和卵泡发育的超声记录。我们将描述月经周期过程中循环性类固醇的特征。此外，我们还将在这组人中检查身体成分、骨密度和结构。我们还将收集血清，以便我们可以在这个队列中观察胰岛素抵抗、心血管风险、DNA和血清的蛋白质组学。我们建议建立有规律月经周期历史的黑人女性性激素水平的标准数据，并将其与白人女性进行比较。将对体重、身高和BMI正常的女性进行连续三个月经周期的研究。这些数据将成为我们的主要结果。我们还建议测量肥胖黑人和白人女性的性激素水平，收集未来的血清，这样我们就可以研究胰岛素抵抗、心血管风险、DNA和血清蛋白质组学，并计算所有组的骨密度和结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to characterize the steroid level profile throughout the menstrual cycle in Black females compared to White females, and to monitor the effects of obesity and race on sex steroid levels and bone mineral density and architecture. There appears to be strong and increasing evidence that there are fundamental differences in circulating sex steroid levels, which contribute to a multitude of end organ changes in Blacks. We propose to document these in greater detail, above all accounting for differences in obesity between racial groups. Additionally there has been nothing but descriptive studies to date, and little exploration of potential mechanisms. In this study 200 normally cycling females of Black and White origin will be studied. We will intensively study the menstrual cycle with daily urinary collections, and for one cycle blood draws and ultrasound documentation of follicular development. We will characterize circulating sex steroid during the course of the menstrual cycle. Additionally we will examine body composition and bone mineral density and architecture in this group. We will also collect serum so that we can look at insulin resistance, cardiovascular risks, DNA, and serum for proteomics in this cohort. We propose to establish normative data for sex hormone levels in Black women with a history of regular menstrual cycles and compare these with their White counterparts. Three consecutive menstrual cycles will be studied in women who are of normal body weight, height, and BMI. These data will serve as our primary outcome. We also propose to measure sex hormone levels in obese Black and White women, collect serum for the future so we can look at insulin resistance, cardiovascular risks, DNA and serum proteomics, and in all groups bone mineral density and architecture will be calculated.