EARLY MARKERS FOR BREAST CANCER RISK IN NIPPLE ASPIRATE FLUID

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。背景：近年来，乳头抽吸液（NAF）被认为是乳腺癌风险标志物的潜在来源。NAF的主要成分是蛋白质，包括各种前列腺调节蛋白，以及通常已知的癌症标志物，如前列腺特异性抗原（PSA）和癌胚抗原（CEA）。然而，目前还没有确定的方法来确定与乳腺癌风险相关的特定NAF蛋白质谱。 具体目标和程序：NAF的蛋白质谱将通过最先进的技术进行分析，包括二维凝胶电泳（2-D凝胶）和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）。这两种技术的结合使用使我们能够研究低风险健康女性的全球蛋白质谱，这些方法具有时间和成本效益，并且可以很容易地适应临床应用。 实验设计：NAF蛋白谱将在三组女性中进行研究和比较：40名无乳腺癌家族史的健康女性，40名一级亲属诊断为乳腺癌的健康女性，以及40名新诊断的乳腺癌女性，包括治疗前的单侧乳腺癌。 意义：本研究的最终目的是发现和分类NAF蛋白，这些蛋白是乳腺癌风险的早期标志物。在NAF中识别乳腺癌风险标志物可能会彻底改变乳腺癌检测，因为它可以进行无限次和非侵入性的，从而为我们提供了一个工具，前瞻性地跟踪一般人群，并更频繁地跟踪高风险人群。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brief Background: In recent years, nipple aspirate fluid (NAF) has been considered as a potential source for breast cancer risk markers. The main components of NAF are proteins, including various hormone-regulated proteins, and generally known cancer markers such as prostate specific antigen (PSA) and carcinoembryonic antigen (CEA). Currently, however, there is no established way to identify the specific NAF protein profiles associated with breast cancer risk. Specific Aims and Procedures: Protein profiles of NAF will be analyzed by state-of-the-art techniques, including two-dimensional gel electrophoresis (2-D gel) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The combined use of these two technologies has enabled us to study global protein profiles in low risk healthy women and these methods are time- and cost-efficient and could be easily adapted for clinical applications. Experimental Design: The NAF protein profiles will be studied and compared among three groups of women: 40 healthy women with no family history of breast cancer, 40 healthy women with first-degree relatives diagnosed with breast cancer, and 40 women with newly diagnosed breast cancer, including those with unilateral breast cancer, before treatment. Significance: The ultimate purpose of this study is to discover and catalog NAF proteins that are early markers for breast cancer risk. Identification of breast cancer risk marker in NAF may revolutionize breast cancer detection because it can be performed an unlimited number of times and non-invasively and thus provides us a tool to follow prospectively the general population and follow more frequently the higher risk populations.