HRT AND PROTEIN EXPRESSION IN POSTMENOPAUSAL WOMEN

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。2.1 研究假设蛋白质组学评价健康绝经后妇女的血清和血浆开始HRT之前和之后将测试的假设，即蛋白质差异表达的外源性HRT管理的结果。还将检验仅用雌激素处理的组与用组合（CEE + MPA）HRT处理的组之间蛋白质差异表达的假设。 2.2 明确健康绝经后妇女HRT前后血清和血浆蛋白质的种类。使用差异分析来识别血清和血浆蛋白表达为HRT暴露的函数，通过比较：o在开始HRT之前和之后一个月的妇女组内，和o在两组妇女之间，通过使用单独的雌激素与联合HRT方案分层。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 2.1 Study Hypotheses Proteomic evaluation of the serum and plasma of healthy postmenopausal women before and after initiating HRT will test the hypothesis that proteins are differentially expressed as a result of exogenous HRT administration. The hypothesis that proteins are differentially expressed between groups treated with estrogen-only versus those treated with combination (CEE + MPA) HRT will also be tested. 2.2 Specific Aims " To define the protein catalogue of serum and plasma of healthy postmenopausal women before and after initiation of HRT. " To use differential analysis to identify serum and plasma proteins expressed as a function of exposure to HRT, by comparing: o Within the group of women, before and one month after initiation of HRT, and o Between two groups of women, stratified by use of estrogen-only versus combination HRT regimens.