ESSENTIAL AND NON-ESSENTIAL ELEMENT BIOAVAILABILITY FROM SUBSISTENCE FOODS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。野生动物毒理学实验室已获得并安装了主要设备，并培训了两名技术人员（Knott女士和Swor女士）和两名学生（Kirk女士和Moses女士）如何使用Perkin Elmer AA800原子吸收分光光度计、Brooks Rand汞分析仪（总汞和单甲基汞）以及与健康评估（影响）相关的其他设备。在Gerald Bratton博士的指导下，与Texas A&M University （College Station）微量元素实验室建立了一个QA/QC项目，并对北极狐（Alopex lagopus）喂养试验中未来食物（有蹄类、海豹等）的大量样品进行了必要和非必需元素的分析。Sara Moses女士领导2005年1月开始的QA/QC工作。海洋哺乳动物组织分析将成为美国国家标准与技术研究院（NIST） QA/QC项目的一部分。在实验室主任（Swor女士）和PI的指导下，所有人都在实验室建立了基本的健康和免疫状况测定方法。这项研究将于2005年6月捕获北极狐，并于今年夏天开始饲养试验。我们将确定必需元素（Cu、Se、Zn）和非必需元素（Cd、Hg）的总体生物利用度，以及从这些选择的自给食物中富集碳和氮的稳定同位素。从长期来看，我们将确定北极狐模型是否适合实验室研究，以解决北极阿拉斯加人类和野生动物食物网中与污染物和传染物有关的免疫、生殖和疾病传播问题。实验室继续招聘一名博士后助理。一个前景仍在威斯康辛大学麦迪逊分校的博士项目中，可能明年（2006年）可用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Wildlife Toxicology Laboratory has acquired and installed major equipment and trained two technicians (Ms. Knott and Ms. Swor) and two students (Ms. Kirk and Ms. Moses) on the use of the Perkin Elmer AA800 Atomic Absorption Spectrophotometer, Brooks Rand Mercury Analyzer (total and monomethyl) and other equipment associated with the health assessment (impacts). A QA/QC program was established with the Trace Elements Laboratory at Texas A&M University (College Station) under the guidance of Dr. Gerald Bratton, and numerous samples of future food items (ungulates, seals, etc.) for the arctic fox (Alopex lagopus) feeding trial were analyzed for essential and non-essential elements. Ms. Sara Moses is leading this QA/QC effort that was initiated January 2005. The marine mammal tissue analyses will be a part of a QA/QC program with the National Institute of Standards and Technology (NIST). Basic health and immune status assays are being established by all in the laboratory under the guidance of the Laboratory Manager (Ms. Swor) and the PI. Capture of arctic foxes for this study will occur June 2005 and the feeding trial initiated this summer. We will determine the general bioavailability of essential (Cu, Se, Zn) and non-essential (Cd, Hg) elements and stable isotopes of carbon and nitrogen enrichment to the arctic fox from these select subsistence food items. In the longer term we will determine the suitability of the arctic fox model for laboratory studies to address immune, reproductive and disease transmission issues related to contaminants and infectious agents in the arctic Alaska human and wildlife food web. The laboratory continues to recruit for a Postdoctoral assistant. One prospect remains in a Ph.D. program at the University of Wisconsin Madison and could be available next year (2006).