INBRE-2 CHEMICAL ECOLOGY OF THE PISCIVORE GUT:

INBRE-2 食鱼肠道的化学生态学：

• 批准号: 8167416
• 负责人: Todd M. O'Hara
• 金额: $ 7.09万
• 依托单位: UNIVERSITY OF ALASKA FAIRBANKS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167416
• 关键词: Area; Biological Availability; Blood; Chemicals; Colorado; Computer Retrieval of Information on Scientific Projects Database; Ecology; Funding; Gastrointestinal Contents; Gastrointestinal tract structure; Grant; Institution; Intestines; Laboratories; Liver; Location; Mercury; Metabolic Biotransformation; Molecular; Parasites; Research; Research Personnel; Resources; Source; Tissues; Toxic effect; Toxicology; United States National Institutes of Health; Universities; Weight; feeding; gastrointestinal; stable isotope

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Wildlife Toxicology Laboratory (PI Todd O'Hara) and Dr. Lora Ballweber (collaborator) from Colorado State University (molecular parasitolgy) propose a research topic in the area of gastrointestinal "ecology" of Hg distribution, dynamics and biotransformation in the intestine of select carnivores (piscivores) to determine relevance in bioavailability and potential toxicity. We will initially focus on macroparasites for which we will determine the "chemical feeding ecology" using C and N stable isotopes and the concentrations and proportions of total Hg and methyl mercury (MeHg). We then assess the compartments with the gut to determine if Hg is partitioning to specific compartments (including macroparasites). We will determine the "trophic level" of the parasite and determine if this correlates with THg and/or MeHg and compare the Hg concentrations within the parasite to the surrounding gut contents and that of other tissues (gut wall, liver, blood, etc.). Our first question asks; Does the "trophic level", morphometrics, location in the gastrointestinal tract, Se concentration, and genera/species of the macroparasite associate with whole parasite body Hg concentration as total Hg and MeHg as wet weight and dry weight? The second question is: Does the Hg concentration (total Hg and MeHg as wet weight and dry weight) of the gastrointestinal contents vary by location and/or fraction in the gastrointestinal tract and vary from that of the macroparasites (e.g., do the macroparasites biomagnify the Hg and/or biotransform it)?

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 来自科罗拉多州立大学的野生动物毒理实验室(Pi Todd O‘Hara)和合作者Lora Ballweber博士(分子寄生虫学)提出了一项关于汞在部分肉食动物(鱼食动物)肠道中的分布、动态和生物转化的胃肠“生态学”领域的研究课题，以确定生物利用度和潜在毒性之间的相关性。我们首先将重点放在大型寄生虫上，我们将使用碳和氮稳定同位素以及总汞和甲基汞(MeHg)的浓度和比例来确定这些寄生虫的“化学摄食生态”。然后，我们用肠道评估这些间隔，以确定汞是否分配到特定的间隔(包括大型寄生虫)。我们将确定寄生虫的“营养水平”，并确定这是否与三氢汞和/或甲基汞相关，并将寄生虫内的汞浓度与周围肠道内容物和其他组织(肠壁、肝脏、血液等)的汞浓度进行比较。我们的第一个问题是：大型寄生虫的“营养水平”、形态测量、在胃肠道中的位置、硒浓度和属/种是否与整个寄生虫体内汞浓度(以总汞表示)和甲基汞(以湿重和干重表示)有关？第二个问题是：胃肠道内容物的汞浓度(总汞和甲基汞的湿重和干重)是否因胃肠道的位置和/或比例而不同，并与大型寄生虫的汞浓度不同(例如，大型寄生虫是否对汞进行生物放大和/或生物转化)？