DE PEDIATRIC COBRE: ALTERATIONS AND RENOVATIONS CORE

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。位于特拉华州威尔明顿的阿尔弗雷德·I·杜邦儿童医院的生命科学中心(LSC)是内穆斯资助的核心设施，旨在使用人类疾病的动物模型进行生物医学研究。该实体工厂包括大约7000平方英尺的研究实验室、维护和动物饲养设施。除了储藏室和自动清洁室外，还有10个动物拘留室、4个湿实验室或小动物程序室、1个大手术室、1个成像和程序室和1个放射室。该中心的工作人员包括一名经理、两名动物饲养员、一名外科服务技术员和一名主治兽医。现有的实体工厂位于一座单独的建筑中。实体工厂正在老化，除了需要扩大空间外，迫切需要升级电力系统和暖通空调系统等关键基础设施组件。此外，位于研究大楼一楼的LSC附属设施由两个小房间(G46/48)组成，目前无法用于生存手术。LSC上一次重大升级是在20世纪80年代末。自那以后，随着使用LSC的研究人员的数量和范围的增加，Nemour生物医学研究显著增长。下面列举了为支持本RFA而提出的设施修改建议。拟对设施进行翻新和改建。大型动物拘留室和储藏区。塞鲁博士和津田博士的项目需要使用大型动物(绵羊和/或猪)。为了满足这些需求，目前的LSC通用储藏室(109)将进行改装，以满足动物的住房和储存要求。对109号房间的翻修将包括增加一面隔墙，以产生两个独立的房间。其中一个房间将被标记为109A，并将继续用于LSC存储；109B房间将成为大型动物的收容室/收容室。B.大型动物康复手术套装。Theroux博士和Tsuda博士提出的项目需要建立一个动物外科套间，以满足覆盖物种的生存外科设施的要求。LSC附属设备，G46/48室，目前用于对大型动物进行非存活手术。目前，G46/48的面积约为500平方英尺。翻新核心的目标是对我们现有的间隔室设施(内穆尔生命科学中心)进行重大升级。如上所述，生命科学中心是由Nemour资助的核心设施，旨在利用人类疾病的动物模型进行生物医学研究。这一工作人员设施目前满足了许多关于啮齿动物和泻形动物的研究的需要。我们预计，儿科研究中心将显著增加对空间的需求，并将在内穆尔生物医学研究所内开始使用新的动物模型。虽然动物设施足以满足当前小动物工作的需要，但它不能满足需要或将需要对小动物进行专门生理学研究的几名研究人员的需求，也不能提供足够的大动物内脏或程序区，这对于开发与儿童相关的动物模型的转化研究计划至关重要。计划中的翻修将创建一个大型动物拘留室、一个用于大型动物生存手术的程序室和一个用于小动物生存手术的程序室。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Life Science Center (LSC) at the Alfred I duPont Hospital for Children in Wilmington, Delaware is a Nemours-funded core facility designed for conducting biomedical research using animal models of human disease. The physical plant encompasses approximately 7,000 sq ft of research laboratory, maintenance and animal holding facilties. There are 10 animal holding rooms, 4 wet laboratories or small animal procedure rooms, 1 major surgery room, 1 imaging and procedure suite, and an irradiation room, in addition to storage rooms and an automated cleaning room. Staffing of the center includes a manager, two animal caretakers, a surgical services technician and an attending veterinarian. The existing physical plant is located in a separate building. The physical plant is aging and in addition to the need for expanded space, upgrades to critical infrastructure components such as the electrical system and the HVAC system are critically needed. In addition, there is an LSC Annex consisting of two small rooms (G46/48) located on the ground floor of the Research building and currently unable to be utilized for survival surgery. The LSC had its last significant upgrade in the late 1980s. Nemours Biomedical Research has grown significantly since then, with escalation in the number and scope of researchers utilizing the LSC. Proposed facility modifications in support of this RFA are enumerated below. PROPOSED RENOVATIONS AND ALTERATIONS TO FACILITIES A. Large animal holding room and storage area. The projects of Drs. Theroux and Tsuda require the use of large animals (sheep and/or pigs). To accommodate these needs, the current LSC general storage room (109) will be modified to meet animal housing and storage requirements. Renovations to Room 109 will include the addition of a dividing wall to produce two separate rooms. One room will be labeled as 109A and will remain in use for LSC storage; Room 109B will become a holding/housing room for large animals. B. Recovery surgery suite for large animals. Projects proposed by Drs. Theroux and Tsuda necessitate the establishment of an animal surgery suite meeting the requirements of a survival surgery facility for covered species. The LSC Annex, Room G46/48, is currently used to perform non-survival surgery on large animals. The square footage in G46/48 at present is approximately 500 sq ft. The objective of the Renovation Core is to provide a significant upgrade to our existing vivarium facilities (Nemours Life Science Center). As noted above, the Life Science Center is a Nemours-funded core facility designed for conducting biomedical research using animal models of human disease. This staff facility currently meets the needs of many studies involving rodents and lagomorphs. We anticipate that the Center for Pediatric Research will significantly increased demands for vivarium space and will initiate the use of new animal models within Nemours Biomedical Research Institute. While the animal facility is sufficient for current small animal work, it does not meet the needs of several researchers who require or will require specialized physiological studies in small animals, nor does it provide sufficient large animal vivarium or procedure areas that will be essential for developing translational research programs with child-relevant animal models. The planned renovations will create a large animal holding room, a procedure room for survival surgery on large animals and a procedure room for survival surgery on small animals.