A respiratory probiotic to combat infection by Actinobacillus pleuropneumoniae in the pig.

一种呼吸道益生菌，可对抗猪的胸膜肺炎放线杆菌感染。

• 批准号: BB/E527171/1
• 负责人: Andrew Rycroft
• 金额: $ 6.94万
• 依托单位: Royal Veterinary College
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE527171%2F1
• 关键词: respiratory; probiotic; combat; infection; Actinobacillus

Pigs suffer a common, natural disease, contagious pleuropneumonia, due to the Gram-negative pathogen A. pleuropneumoniae (App). Some pigs suffer severe acute (fatal) disease and a high proportion harbour the bacteria in the respiratory lymphoid tissues. In-feed antibiotics and vaccination can control the disease but do not remove the pathogen from the herd. As antibiotic use becomes less publicly acceptable, alternative methods will be needed to control endemic disease in the herd. In BBSRC-funded research of genetics of virulence of App we have generated a number of mutants of App. In a new approach to control disease and carriage of App, we will test genetically-defined, non-virulent mutants to competitively exclude App from the respiratory tract and lymphoid tissues of the pig and occupy the ecological niche of the pathogen. A 'designer probiotic' for the respiratory tract represents a new, safe biological control method intended to reduce or eliminate these bacteria in a pig herd. This concept, a novel means of control of endemic respiratory disease in farmed animals, may offer the opportunity for a gradual eradication of this pathogen from the national herd.

猪患有一种常见的自然疾病，传染性胸膜肺炎，由于革兰氏阴性病原体A。胸膜肺炎（App）。一些猪患有严重的急性（致命）疾病，并且呼吸道淋巴组织中有很高比例的细菌。饲料中抗生素和疫苗接种可以控制疾病，但不能从牛群中清除病原体。随着抗生素的使用越来越不为公众所接受，将需要替代方法来控制畜群中的地方病。在BBSRC资助的App毒力遗传学研究中，我们已经产生了一些App突变体。在控制疾病和App携带的新方法中，我们将测试遗传学定义的无毒突变体，以竞争性地将App从猪的呼吸道和淋巴组织中排除，并占据病原体的生态位。用于呼吸道的“设计益生菌”代表了一种新的，安全的生物控制方法，旨在减少或消除猪群中的这些细菌。这一概念是控制养殖动物地方性呼吸道疾病的一种新手段，可能为从国家畜群中逐步根除这种病原体提供机会。

项目成果

Colonisation of pig lymphoid tissues with avirulent Actinobacillus pleuropneumoniae: competitive exclusion and displacement of virulent A. pleuropneumoniae from the respiratory tract?

无毒力胸膜肺炎放线杆菌在猪淋巴组织中的定植：竞争性排除和置换呼吸道中的强毒力胸膜肺炎放线杆菌？

• 发表时间: 2012
• 作者: Andrew N Rycroft

Necrosis from needlestick injury with live Actinobacillus pleuropneumoniae porcine vaccine.

猪胸膜肺炎放线杆菌活疫苗导致针刺伤坏死。

• DOI: 10.1136/bmj.d6261
• 发表时间: 2011
• 期刊: BMJ (Clinical research ed.)
• 作者: Rycroft AN

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae.

• DOI: 10.1186/s13567-017-0502-x
• 发表时间: 2018-01-09
• 期刊: Veterinary research
• 影响因子: 4.4
• 作者: Antenucci F;Fougeroux C;Deeney A;Ørskov C;Rycroft A;Holst PJ;Bojesen AM
• 通讯作者: Bojesen AM