In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae.
In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae.
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DOI：
10.1186/s13567-017-0502-x
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发表时间：
2018-01-09
影响因子：
4.4
通讯作者：
Bojesen AM
中科院分区：
文献类型：
--
作者：
Antenucci F;Fougeroux C;Deeney A;Ørskov C;Rycroft A;Holst PJ;Bojesen AM
胸膜肺炎放线杆菌（Actinobacillus pleuropneumoniae，A.胸膜肺炎（Pleuropneumoniae）是一种革兰氏阴性细菌，其代表猪胸膜肺炎的主要原因，给全世界的畜牧业造成重大的经济损失。A.作为大多数革兰氏阴性细菌，胸膜肺炎从其外膜（OM）分泌囊泡，因此定义为外膜囊泡（OMV）。由于它们与OM的抗原相似性，OMV已成为疫苗学中有前途的工具。在这项研究中，我们描述了几种疫苗原型的体内试验，用于预防所有已知的A。胸膜肺炎血清型。采用先前鉴定的候选疫苗，重组蛋白ApfA和VacJ，单独施用或与OMV以各种组合施用，作为疫苗接种策略。我们的数据显示，在疫苗制剂中添加OMV显著增加了免疫动物中针对ApfA和VacJ的特异性IgG滴度，证实了先前假设的OMV作为佐剂的潜力。不幸的是，所产生的抗体应答并没有转化为针对A的有效保护。因此，在胸膜肺炎感染中，没有免疫组在攻击后显示出比对照显著更低程度的病变。有趣的是，事实恰恰相反，因为IgG滴度最高的动物也是肺部病变最广泛的动物。这些结果为A.胸膜肺炎病原性，表明来自宿主免疫应答的抗体介导的细胞毒性可能在通常与A.胸膜肺炎感染。
Actinobacillus pleuropneumoniae (A. pleuropneumoniae) is a Gram-negative bacterium that represents the main cause of porcine pleuropneumonia in pigs, causing significant economic losses to the livestock industry worldwide. A. pleuropneumoniae, as the majority of Gram-negative bacteria, excrete vesicles from its outer membrane (OM), accordingly defined as outer membrane vesicles (OMVs). Thanks to their antigenic similarity to the OM, OMVs have emerged as a promising tool in vaccinology. In this study we describe the in vivo testing of several vaccine prototypes for the prevention of infection by all known A. pleuropneumoniae serotypes. Previously identified vaccine candidates, the recombinant proteins ApfA and VacJ, administered individually or in various combinations with the OMVs, were employed as vaccination strategies. Our data show that the addition of the OMVs in the vaccine formulations significantly increased the specific IgG titer against both ApfA and VacJ in the immunized animals, confirming the previously postulated potential of the OMVs as adjuvant. Unfortunately, the antibody response raised did not translate into an effective protection against A. pleuropneumoniae infection, as none of the immunized groups following challenge showed a significantly lower degree of lesions than the controls. Interestingly, quite the opposite was true, as the animals with the highest IgG titers were also the ones bearing the most extensive lesions in their lungs. These results shed new light on A. pleuropneumoniae pathogenicity, suggesting that antibody-mediated cytotoxicity from the host immune response may play a central role in the development of the lesions typically associated with A. pleuropneumoniae infections.
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影响因子：
9.4
作者：
Bossé JT;Li Y;Sárközi R;Gottschalk M;Angen Ø;Nedbalcova K;Rycroft AN;Fodor L;Langford PR
通讯作者：
Langford PR
影响因子：
2.4
作者：
HANNAN, PCT;BHOGAL, BS;FISH, JP
通讯作者：
FISH, JP
影响因子：
6.4
作者：
Chiers, K;van Overbeke, I;Haesebrouck, F
通讯作者：
Haesebrouck, F
影响因子：
2.5
作者：
Aarestrup, Frank M.;Duran, C. Oliver;Burch, David G. S.
通讯作者：
Burch, David G. S.
影响因子：
2.1
作者：
Negrete-Abascal, E;García, RM;de la Garza, M
通讯作者：
de la Garza, M